前庭损伤后脑干相关神经元C-FOS的表达  

C-FOS expression in the related brainstem neurons after vestibular lesion

作　　者：蒋子栋[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院耳鼻咽喉科,北京100730

出　　处：《中华耳科学杂志》2006年第4期294-297,共4页Chinese Journal of Otology

基　　金：香港大学郑裕彤奖助金资助项目;国家人事部归国人员启动基金资助项目。

摘　　要：目的观察大鼠内耳前庭损伤后,在静止和角加速度刺激状态下双侧前庭神经核C-FOS阳性表达的改变,初步探讨前庭代偿机制。方法将60只SD大鼠分为5组(12只/组):(1)无手术组;(2)单耳前庭神经切断手术组;(3)单耳前庭神经切断手术对照组;(4)双耳前庭神经切断手术组;(5)双耳前庭神经切断手术对照组,每组一半动物给予角加速度刺激。用免疫组化ABC法观察C-FOS在前庭神经核、舌下神经前置核的神经元表达的改变。结果在静止状态下,正常鼠和手术对照组鼠的双侧前庭神经核、舌下神经前置核无C-FOS阳性神经元;给予旋转刺激后,双侧前庭神经核、舌下神经前置核皆有C-FOS阳性神经元。单耳手术组术后24小时,同侧前庭神经内核、对侧舌下神经前置核有C-FOS阳性神经元,旋转刺激后双侧前庭神经核、舌下神经前置核皆有C-FOS阳性神经元。双耳手术组24小时,在静止状态和角加速度刺激状态下,双侧前庭神经核、舌下神经前置核皆无C-FOS阳性神经元。结论一侧前庭损伤后,同侧前庭神经内核、对侧舌下神经前置核C-FOS阳性神经元在前庭代偿早期可能起启动突触联系的作用。Objective In order to study the mechanism of vestibular compensation, the changes of Fos expression in the brainstem of the vestibular deafferentation rats were observed both in the situation of static status and following angular acceleration stimulation. Methods Sixty SD rats were randomly divided into 5 groups （n=12 each group）, including control （labyrinthine intact）, complete unilateral vestibular deafferentation （UVD） group, simultaneous complete bilateral vestibular deafferentation （BVD） group, sham UVD group and sham BVD group. Each group was further divided into subgroups （6 rats each）： in static status or undergoing angular acceleration stimulation. Immunohistochemistry was used for Fos protein labeling. Results Prominent Fos expression in the ipsilesional medial vestibular nucleus （MVN） and the contralesional prepesitus hypoglossi （PrH） at 24 hours after unilateral vestibular deafferentation, higher Fos staining, however, can be detected in both sides of MVN and PrH when unilateral vestibular neurectomy rats underwent angular acceleration stimulation. The same as that of labyrinthine intact rats in static status, no Fos expression in MVN and PrH of both sides of simultaneous bilateral vestibular deafferentation rats whether they underwent rotation or not. Conclusion Our results suggest that the integrity of unilateral vestibular afferentation plays an important role in vestibular compensation and Fos marked ipsilesional MVN act as a group of specific functional neurons connecting contralesional periphery with ipsilesional vestibular nuclear complex.

关 键 词：前庭神经切断术 前庭代偿 C—FOS 

分 类 号：R339.16[医药卫生—人体生理学] R322.923[医药卫生—基础医学]