NKG2D及其配体RAE-1、H60在移植物抗肿瘤中的作用  

Effects of NKG2D and Its Ligands RAE-1 and H60 on Graft-versus-Tumor Response

作　　者：李晓峰[1] 陈强[2] 叶韵斌[2] 范南峰[2] 陈明水[2] 李洁羽[2] 陈慧菁[2] 陈淑萍[2] 周智锋[2] 

机构地区：[1]福建省泉州市中医院肿瘤科,泉州362000 [2]福建省肿瘤医院内科,福州350014

出　　处：《中国实验血液学杂志》2007年第1期160-164,共5页Journal of Experimental Hematology

基　　金：福建省自然科学基金资助(编号C0310035);福建省医学创新课题(编号2003-CX-16);福建省科技厅重点科技项目(编号2006Y0011)

摘　　要：为探讨NKG2D及其配体RAE-1、H60在单倍体相合(haploidentical)造血干细胞移植介导的移植物抗肿瘤效应中的作用,以皮下接种H22细胞的BABL/c×C57BL/6杂交F1代(CB6F1)小鼠为受鼠,以C57BL/6小鼠的骨髓+脾细胞为供者细胞,分别用抗CD90.2、NK1.1和NKG2D单克隆抗体清除供者T细胞、NK细胞和封闭NKG2D受体,进行单倍体相合细胞移植,观察移植后的抗肿瘤效应,并以半定量RT-PCR法检测移植后小鼠肿瘤组织RAE-1、H60的mRNA表达水平。结果发现:清除供者T细胞、NK细胞和封闭NKG2D的移植均导致移植物抗肿瘤效应的下降,MHC单倍体相合细胞移植后肿瘤细胞RAE-1、H60的mRNA表达水平上升。结论:NKG2D及其配体RAE-1、H60在移植物抗肿瘤效应中的起着重要的作用。The study was purposed to explore the effects of NKG2D receptor and its ligands RAE-1 and H60 on graft-versus-tumor （GVT） response induced by MHC haploidentical bone marrow/spleen cell transplantation. Female （BALB/c × C57BL/6） F1 mice （ CB6F1 ,H-2K^b/d） inoculated with H22 cells to develop a solid tumor model were the recipients, and bone marrow mixed with spleen cells of the healthy male C57BL/6（ H-2K^b） mice were the donor cells. GVT response was observed after transplantation that from donor cells T and NK cells were purged with anti-CD3 and anti-NK monoclonal antibody, and the NKG2D receptor was blocked with anti-NKG2D monoclonal antibody, the expression levels of RAE-1 and H60 mRNA in tumor tissue were measured by means of semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） at different time points after transplantation. The results showed that the GVT response of transplantation was reduced after in vitro depletion of T and NK cells or blocking NKG2D receptor in donor cells of the graft, the expression levels of RAE-1 and H60 mRNA in tumor tissue increased after transplantation of haploidential bone marrow mixed with spleen cells. It is concluded that NKG2D and its ligands RAE-1 and H60 may play important roles in GVT response.

关 键 词：NKG2D RAE-1 H60 移植物抗肿瘤效应 

分 类 号：R318.06[医药卫生—生物医学工程] R730.51[医药卫生—基础医学]