Localization of NOS-like protein in guard cells of Vicia faba L.and its possible function  

作　　者：LIU Xin WANG YouQun JIA WenSuo LOU ChengHou ZHANG ShuQiu 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beiiing 100094, China [2]Department of Life Science, Lai Yang Agricultural College, Qingdao 266109, China

出　　处：《Chinese Science Bulletin》2007年第1期84-90,共7页

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30370141)

摘　　要：Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of ni- tric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS in- hibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO genera- tion from NOS pathway is the main pathway in the stress and JA responses. The pharmacological ex- periment showed that increasing the Ca2+ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca2+-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses.Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of nitric oxide synthase （NOS）-Iike proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide （NO） and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS inhibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO generation from NOS pathway is the main pathway in the stress and JA responses. The pharmacological experiment showed that increasing the Ca^2＋ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca^2＋-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role In plant stress responses.

关 键 词：免疫细胞 氧化一氮 连位化合物 生物化学 

分 类 号：Q26[生物学—细胞生物学]