淋球菌外膜蛋白Porin I多抗的黏附抑制作用研究  

Adhesional inhibiton of polyclonal anti-porin I antibody

作　　者：郑磊[1] 阮黎明[1] 程浩[1] 岑建萍[1] 沈建根[1] 林洁[1] 张行[1] 姚云斌[1] 王琦[1] 陈大方[1] 

机构地区：[1]浙江大学医学院附属儿童医院皮肤科,浙江杭州310003

出　　处：《浙江大学学报（医学版）》2007年第1期78-83,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省卫生厅科研基金(491030-W10001);浙江省科技厅科研基金(491030-J30017)

摘　　要：目的:研究兔抗淋球菌外膜蛋白Porin I(P I)的多克隆抗体阻断淋病奈瑟菌对泌尿生殖道上皮的黏附作用。方法:将自行构建表达的淋球菌GST-P I融合蛋白作为抗原免疫新西兰兔,获得兔抗P I蛋白的多抗血清,纯化后得到P I-IgG抗体。建立淋球菌感染BALB/c小鼠模型,并通过观察阴道黏膜改变、分泌物涂片、冲洗液培养及阴道组织病理变化评价兔抗P I-IgG抗体对淋球菌黏附的影响。结果:经1 m g/m l兔抗P I-IgG处理后3 h再接种淋球菌的BALB/c小鼠生殖道黏膜未见红肿及脓液,分泌物涂片及阴道冲洗液未检及淋球菌,阴道组织病理检查也未见炎症细胞浸润。而低于浓度1 m g/m l及长于3 h兔抗P I-IgG处理的小鼠阴道组织病理可检及炎症细胞,但其它检查结果阴性。结论:纯化的抗淋球菌GST-P I融合蛋白的多克隆抗体,能有效抑制淋病奈瑟菌对小鼠泌尿生殖道上皮的黏附与感染,阻断作用及持续时间与抗体浓度有关。Objective： To investigate the blockness effects of purified polyclonal anti- porin I antibody on N. gonorrhoeae adherence to genitourinary tract epithelia of BALB/c mouse. Methods： Polyclonal anti GST-PI antibody was generated by immunizing rabbit with GST-PI fusion protein which was constructed and expressed by ourselves. The purified immunoglobulin G was obtained by ammonium sulphate deposition and DEAE cellulose chromatography. Mice model of gonorrhea was established. In order to evaluate the effects of PI-IgG on gonococcus adhesion to vagina mucus, the macroscopic and pathological assessing as well as gonococcus culture was employed after gonococcus challenge on PI-IgG immunized mice. Results ： No pus and pathological inflammation were observed on mice vagina mucus treated with 1 mg/ml PI-IgG 3 hours before gonococcus challenge. Gonococcus could not be detected in the smears and washing solutions from vagina. Pathological inflammation was found in mice treated with anti PI-IgG, in which the concentrations were lower than 1 mg/ml or the treated time was longer than 3 hours prior to gonococcus challenge. Conclusion： The purified anti PI-IgG can effectively inhibit the adherence and infection of gonococci to genitourinary tract epithelia of BALB/c mice. In addition, the blocking duration of anti PI-IgG is associated with antibody concentration.

关 键 词：奈瑟球菌 淋病 疾病模型 动物 淋球菌 外膜蛋白I 多克隆抗体 黏附抑制 

分 类 号：R378[医药卫生—病原生物学]