AML1／ETO9a异构体在M2型急性髓系白血病中表达的研究  

Expression of AML1/ETO9a isoform in acute myeloid leukemia-M2 subtype

作　　者：苗雨青[1] 陈子兴[1] 何军[1] 岑建农[1] 鲍晓晶[1] 邱桥成[1] 张东尔 严明[2] 

机构地区：[1]苏州大学附属第一医院、江苏省血液研究所,215006 [2]美国seripps研究所分子和实验医学部

出　　处：《中华血液学杂志》2007年第1期27-29,共3页Chinese Journal of Hematology

摘　　要：目的研究AML1／ETO9a异构体在急性髓系白血病M2型（AML—M2）中的表达。方法应用R带染色体核型分析，结合RT—PCR技术检测各型白血病、骨髓增生异常综合征（MDS）、白血病细胞系及正常人骨髓中AML1／ETO融合基因和AML1／ETO9a异构体的表达。结果在30例初诊AML-M2中有15例AML1／ETO9a异构体表达阳性，同时在20例AML—M2完全缓解患者、18例非M2型急性白血病患者、5例慢性粒细胞白血病（CML）患者、3例MDS患者、3个白血病细胞系（NB4、KG-1、K562）及5份正常骨髓标本中均未检测到AML1／ETO9a异构体。15例AML1／ETO9a异构体阳性患者中有13例同时具有AML1／ETO融合基因及t（8；21），2例仅表达AML1／ETO9a异构体，AML1／ETO融合基因及t（8；21）均未检测到。结论AML1／ETO9a异构体可能与AML—M2相关，并可能与AMLI／ETO融合基因共同参与白血病的发生。Objective To investigate the expression of AML1/ETO9a isoform in the acute myeloid leukemia （AML）- M2 patients. Methods Expressions of AML1/ETO fusion gene and AML1/ETO9a isoform were detected by using reverse transcriptase-polymerase chain reaction （RT-PCR） in leukemia patients, MDS patients, leukemia cell lines and healthy subjects. Karyotype was studied by R-banding technique. Result In 30 newly diagnosed AML-M2 patients 15 were found to express AML1/ETO9a isoform, while the rest including 20 AML- M2CR, 18 other subtypes of AML, 5 chronic myelogenous leukemia（CML） , 3 myelodysplastic syndromes （MDS）, 3 leukemia cell lines （NB4, KG-1, K562） and 5 healthy subjects were AML1/ ETO9a negative. Among the 15 AML/ETO9a isoform expressing cases, 13 were demonstrated t（8 ;21 ） translocation and AML1/ETO expression. Conclusion Isoform AML1/ETOga was correlated to AML/M2 , and it may promote the development of leukemia in combination with the AML1/ETO fusion gene.

关 键 词：白血病 粒细胞 急性 异构现象 融合基因 AML1/ETO 逆转录聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]