染氟成纤维细胞内Ca^(2+)水平的变化及其意义  

Changes and effects of intracellular calcium level in fibroblast induced by fluoride

作　　者：井玲[1] 齐玲[1] 赵志涛[1] 李广生[1] 

机构地区：[1]吉林大学地方病研究所,长春130021

出　　处：《中国地方病防治》2007年第1期14-15,共2页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金资助课题(30471498)

摘　　要：目的研究氟对成纤维细胞Ca2+浓度的影响并探讨其意义。方法应用扫描共聚焦显微技术测定不同染氟条件下(0.0001、1、10mg/LF-)成纤维细胞Ca2+水平的变化。结果染氟1mg/L组成纤维细胞在染氟500s左右时[Ca2+]i水平开始升高,约1500s达到高峰,然后开始下降,3000s左右回到起点位置。结论成纤维细胞染氟1mg/LF-组[Ca2+]i有一过性升高。认为这种[Ca2+]i浓度的变化在影响成纤维细胞中cbfa1和某些生长因子之间的相互关系、促进细胞增殖、分化及成骨表型转换中可能起重要作用。Objective To study the effects of fluoride on the level of Ca^2＋ release in cultured fibroblast. Methods Fibroblasts were cultured and loaded with fluo - 3 - AM. [ Ca^2＋ ] i was measured by fluorescent intensity in each cell with laser confocal microscope. Results In the group of 1 mg/LF- , the intercellular free calcium concentration in fibroblasts increased transitorily. Conclusions The elevation of [ Ca^2＋ ] i in fibroblast may play a important role in regulating the cooperation and restriction among the cbfal and other growth factors ,promoting the proliferation, differentiation, and the expression of osteogenesis phenotype.

关 键 词：氟化物 成纤维细胞株(L929) 细胞内钙 成骨表型 

分 类 号：R599.9[医药卫生—内科学]