bFGF对成骨细胞整合素β1亚单位表达和细胞黏附的影响  

Effects of basic fibroblast growth factor(bFGF) on expression of β1 integrin subunit and adhesion of osteoblast

作　　者：冯立[1] 曹晓建[1] 任永信[1] 杨志明[2] 

机构地区：[1]南京医科大学第一附属医院骨科,江苏南京210029 [2]四川大学华西医院干细胞与组织工程研究室,四川成都610041

出　　处：《南京医科大学学报（自然科学版）》2007年第1期1-5,F0002,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家高技术研究发展计划(863)资助项目(2002AA205011);南京医科大学科技创新基金(CX2003016);南京医科大学第一附属医院人才引进基金

摘　　要：目的:研究碱性成纤维细胞生长因子(bFGF)对成骨细胞整合素β1亚单位表达的影响,探讨纤维黏连蛋白(FN)与碱性成纤维细胞生长因子(bFGF)单独或联合应用时对成骨细胞在生物衍生骨支架上黏附的影响。方法:培养成骨细胞,以bFGF(100ng/ml)刺激,接种于FN(10μg/ml)修饰的生物衍生骨支架上,Westernblot检测bFGF对FN受体整合素β1亚单位表达的影响,MTT检测组间细胞黏附效率差异,电镜观察细胞密度与形态。结果:Westernblot检测发现应用bFGF刺激后,成骨细胞整合素β1亚单位表达增强;MTT检测发现单独应用FN与bFGF均可增加细胞在生物衍生骨上的黏附效率(P<0.01),但二者联合应用时,产生的效应进一步增强,二者存在统计学上的交互作用(P<0.01);电镜观察也证明,二者联合应用时细胞密度、铺展效果均优于单独应用。结论:bFGF可以显著增强成骨细胞FN受体整合素β1亚单位的表达,明显提高成骨细胞在FN修饰生物衍生骨支架上的黏附效率,bFGF与FN对于促进成骨细胞的黏附具有交互作用。Objective：To investigate the effects of basic fibroblast growth factor（bFGF） on the expression of β1 subunit of integrin and to explore the effects of bFGF and fibronection （FN） on osteoblast adhesion on bio-derived bone. Methods：The third generation of osteoblast was treated with bFGF（100 ng/ml） and then seeded on bio-derived bone which had been modified with FN （10 ug/ml）. Expression of β1 subunit of integrin was detected by Western blot. Cell adhesion was measured by MTT assay. Cell density and appearance was observed by scanning electron microscope. Results：The expression of β1 subunit of integrin was significantly enhanced by bFGF. Both FN and bFGF could enhance osteoblast＇s adhesion efficiency on bio-derived bone （P〈 0.01 ）. But the osteoblast＇s adhesion efficiency was significantly strengthened by combined use of FN and bFGF. They had a significant synergistic effect in statistics （P 〈 0.01 ）, Combined use of FN and bFGF had a better effect on cell density and appearancc than solo use when observed with scanning electron microscope. Conelusion：BFGF can significantly enhance the expression of β1 subunit of integrin and significantly strengthen osteoblast＇s adhesion efficiency on bio-derived bone wbich has been modified with FN. BFGF and FN have a significant synergistic effect on nstenblast＇s adhesion.

关 键 词：骨组织工程 碱性成纤维细胞生长因子 整合素 纤维黏连蛋F1 

分 类 号：R318.17[医药卫生—生物医学工程] Q813.1[医药卫生—基础医学]