树突状细胞表面共刺激分子在哮喘小鼠中的作用研究  

Study of costimulatory molecules of dendritic cells in asthmatic mice

作　　者：吴剑卿[1] 许伟[1] 孙芸[1] 殷凯生[2] 

机构地区：[1]南京医科大学第一附属医院老年医学科,江苏南京210029 [2]南京医科大学第一附属医院呼吸内科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第1期31-35,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅自然科学基金资助(03KJD320142)

摘　　要：目的:研究哮喘小鼠骨髓来源树突状细胞(DCs)的表型及共刺激分子在哮喘发病中的影响。方法:用卵清蛋白建立哮喘模型,利用rmGM-CSF和rmIL-4体外诱导骨髓细胞分化为DCs,流式细胞仪检测DCs表面共刺激分子的表达,混合淋巴细胞反应检测DCs刺激同种异体的T淋巴细胞增殖的能力。结果:在两种细胞因子的作用下,从骨髓中可以诱导出大量的DCs;且均表达DCs的表面标志(33D1)。哮喘组DCs表达CD86分子的水平高于对照组,而CD40、CD80在两组之间没有差异;哮喘组与对照组DCs均能强烈刺激同种异体T淋巴细胞的增殖,哮喘组DCs的刺激能力明显强于对照组。结论:哮喘组DCs的抗原递呈能力增强,表明DCs在哮喘的发病中可能起着重要作用。Objective：To evaluate the effects of dendritic cells derived from bone marrow in the pathogenesis of asthma. Methods： A routine model of ovalbumin （OVA）-allergic asthma was established. DCs were developed by incubating bone marrow cells with rmIL-4 and granuloeyte-maerophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the mixed leukocyte reaction. Results： DCs expressing DCs characteristic 33D1 could be developed from bone marrow cells by using rmIL-4 and rmGM-CSF. The stimulating effect of DCs on the proliferation of the lymphocytes was shown in asthmatic and control group, but the effect in asthmatic group was more intensive than that in control group. DCs from asthmatic mice showed different phenotypes of CD86, but there were no differences in the expression of CD80 and CD40 between asthmatic and control group. Conclusion：This study suggests the antigen-presenting function of DCs in asthmatic group is increased and DCs may play an important role in the pathogenesis of asthma.

关 键 词：哮喘 树突状细胞 B7 CD40 混合淋巴细胞反应 

分 类 号：R562.25[医药卫生—呼吸系统]