同基因造血干细胞移植延长同种异体小鼠皮肤移植物存活时间的实验研究  

Prolongation of skin allograft survival in mice by syngenic hematopoietic stem cell transplantation

作　　者：王琳[1] 周景师[1] 赵青川[1] 韩骅[2] 易蔚[1] 王耀春[2] 窦科峰[1] 

机构地区：[1]第四军医大学西京医院肝胆外科全军器官移植中心,西安710032 [2]第四军医大学基础部遗传与发育教研室,西安710033

出　　处：《现代免疫学》2007年第1期45-48,共4页Current Immunology

基　　金：军队十一五医药卫生科技攻关项目

摘　　要：为了研究同基因造血干细胞移植诱导器官移植免疫耐受的可行性。建立小鼠异基因皮肤移植模型,术后2周给予FK506腹腔注射,3周起行全身照射及同基因骨髓移植,观察记录小鼠和移植物存活情况,以流式细胞检测受体GFP嵌合表达,混合淋巴细胞反应、迟发型超敏反应检测诱导耐受的特异性和效能。实验组小鼠移植物存活时间达(29.14±4.92)d,显著长于对照组(P<0.05);GFP在BMT后4周、6周嵌合程度达到82%、91%;实验组MLR、DTH结果与对照组差异显著,提示诱导耐受具有高度特异性和高效性。同基因造血干细胞移植联合免疫抑制剂治疗可以有效诱导小鼠皮肤移植的免疫耐受。To investigate the mechanism of olerance of organ transplantation following syngenic hematopoietic stem cell transplantation（HSCT）, the mouse model was constructed by allogenic skin transplantation, and FK506 was injected into peritoneal cavity 2 weeks later. Three weeks later, the treated group was exposed to total body irradiation and accepted the syngenic bone marrow transplantation from GFP C57BL/6 transgenic mice . The viability of chimeric mice and graft were observed constantly. The GFP expression in peripheral blood was analyzed by FACS; the specification of induced tolerance was detected by MLR. It was found that the survival time of mouse from treated group was （29. 14!4.92） d, significantly longer than the control group（P〈0. 05）. The expression of GFP following BMT was 82 % （4w） and 91% （6w） respectively. The outcome of MLR and DTH for the treated group had significant difference to the control, P〈0.05. It is concluded that syngenic HSCT combined with immunosuppressive treatment may induce tolerance to skin transplantation.

关 键 词：皮肤移植 骨髓移植 造血干细胞移植 免疫耐受 GFP 

分 类 号：R392[医药卫生—免疫学]