自由基清除剂依达拉奉对脓毒性休克家兔的治疗作用  

Therapeutical efficacy of free radical scavenger edaravone on septic shock rabbits

作　　者：霍小萍[1] 殷晓进[1] 刘良明[2] 刁有芳[2] 陈家慧[1] 李萍[2] 刘建仓[2] 徐强[3] 

机构地区：[1]江苏先声药物研究有限公司,江苏南京210042 [2]中国人民解放军第三军医大学附属大坪医院野战外科研究所第二研究室,重庆400042 [3]南京大学生命科学院,江苏南京210093

出　　处：《中国新药与临床杂志》2007年第1期17-22,共6页Chinese Journal of New Drugs and Clinical Remedies

基　　金：江苏省博士后科研资助计划(No.0401079B)

摘　　要：目的：观察依达拉奉(ED)对脓毒性休克家兔血气、血生化指标及动物存活率的影响。方法：动物脓毒性休克后1 h用3个剂量ED(1．6,0．8,0．4 mg·kg^(-1))治疗,测定4个时相点动脉血气及酸碱度,并测血乳酸(L)、乳酸脱氢酶(LDH)、过氧化氢酶(CAT)、丙二醛(MDA)、超氧化物歧化酶(SOD)、肌酐(Cr)及尿素氮(BUN)含量。观察24 h时动物存活情况。结果：家兔脓毒性休克4 h后出现代谢性酸中毒合并呼吸性酸中毒,L、LDH、BUN明显升高,SOD活性明显降低,动物死亡率为70％。不同剂量ED治疗后可改善血气、酸碱平衡紊乱,增强SOD和CAT活性,降低LD、MDA浓度及LDH活性。治疗后3个剂量组动物死亡率分别为0,0和33％,明显低于模型组(P<0．05)。结论：ED能增强脓毒性休克机体的抗氧化能力,降低脓毒性休克死亡率。AIM： To investigate the effects of edaravone （ED）, a novel free radical scavenger, on arterial blood gases, some plasma biochemical parameters, and survival rate in rabbits with septic shock. METHODS： After treatment with three different doses of ED （1.6, 0.8, 0.4 mg·kg^-1） to rabbits suffered septic shock, changes of arterial blood gases, blood acid-base scale, plasma levels of lactic acid, MDA, Cr and BUN; activities of LDH, CAT and SOD were determined in four different time phases. The mortality of animals in 24 h was observed. RESULTS： Four hours after septic shock, metabolic acidosis and respiratory alkalosis occurred, the blood levels of lactic acid, BUN and activity of LDH increased significantly but the activity of SOD reduced markedly; with death rate up to 70 %. However, following the treatment with different doses of ED, the degrees of the derangement of blood gases and acid-base equilibrium were improved with enhancement of the plasma activities of SOD and CAT and decrease of the plasma levels of LD, MDA and activity of LDH. The death rate in treated groups was signifcantly lower than that of the increase the protection that of organ and tissue against damage model group （P 〈 0.05）. CONCLUSION ： ED can free radical-mediated injury, decrease the secondary of reactive oxygen species, and recover the metabolic derangement of septic shock.

关 键 词：依达拉奉 自由基清除剂 休克 脓毒性 

分 类 号：R965[医药卫生—药理学] R605.971[医药卫生—药学]