直流电场对鸡胚绒毛尿囊膜血管生成作用的研究  

Effect of Direct Electric Fields on Angiogenesis of CAM

作　　者：邵阳[1] 王永堂[2] 高洁[2] 罗治斌[2] 

机构地区：[1]川北医学院附属医院,四川南充637000 [2]第三军医大学大坪医院野战外科研究所,重庆400042

出　　处：《中国医学物理学杂志》2007年第1期36-37,40,共3页Chinese Journal of Medical Physics

基　　金：国家自然科学基金资助(30300075);军队"十五"青年基金资助(01Q106)

摘　　要：目的:探讨直流电场(electric fields,EF)对血管生成的影响及作用机制。方法:于发育第8天的鸡胚绒毛尿囊膜(chick embryo chorioallantoic membrane,CAM)上植入明胶海绵载体,其中加载不同强度EF,计数长入载体的血管和载体内的微血管密度(microvascular density,MVD)。结果:150 mV/mm、200 mV/mm组在CAM上长入载体边缘的血管数和MVD与对照组间的有显著差别(p<0.01)。结论:强度为150 mV/mm、200 mV/mm的直流电场能促进CAM的血管生成。Objective： The study was to reveal the effect of direct electric fields on angiogenesis of chick embryo chorioallantoic membrane（CAM）. Methods： Gelatin sponges were implanted on top of CAM, when chick embryos developed on the 8th day, vessels which towards gelatin and microvascular density（MVD） in gelatin were counted on the 12th day. Results： On CAM model, numbers of vessels at the sponge-CAM boundary and MVD were significantly different between group of 150 mWmm, 200 mV/mm and group of saline （p〈 0. 01）. Conclusions： The study has shown that direct electric fields, 150 mV/mm and 200 mV/mm, could promote the angiogenesis on CAM model.

关 键 词：直流电场 血管生成 鸡胚绒毛尿囊膜 

分 类 号：R322.1[医药卫生—人体解剖和组织胚胎学] R363.1[医药卫生—基础医学]