^(125)I标记消脂素的制备研究  

Study on Preparation of ^(125)I Iodination Labelling of Leptin

作　　者：张中书[1] 许瑞吉[1] 王国洪[1] 王筱劼[1] 

机构地区：[1]南京军区南京总医院放免室,江苏南京210002

出　　处：《标记免疫分析与临床》2006年第4期236-237,247,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：以间接碘标记法与直接碘标记法标记消脂素(leptin,LEP),并分别建立LEP的RIA进行方法学的比较。间接碘标记法先以氯胺T法标记对羟基苯丙酸琥珀酰亚胺脂(BH试剂)制备125I-BH,并与LEP进行联结制备125I-LEP。125I-LEP放射性比活度为1.43 MBq/μg,125I的总标记率为37.8%,125I-LEP的最大结合率(Bmax)为97.5%。所建立的RIA的NSB为6.7%,零计量管(B0)的结合率(B/T)为43.7%,灵敏度为0.25μg/L,高、中、低三种浓度的样品批内CV为7.8%,批间CV为12.9%,回收率为96.4%~103.2%,与TSH、LH、FSH、PRL、GH及Ins等均无交叉反应,与Linco的药盒进行临床测试对比亦显示良好的线性关系,r为0.951,以间接碘标记法标记的125I-LEP在45天内其标准曲线的稳定性较直接法标记的125I-LEP有较大的提高,能满足临床工作的需求。^125I-LEP was prepareded by ^125I with indirect iodination and direct iodination respectively, to develop RIA for method comparison. For indirect iodination, BH agent was iodinated by ch-T , then conjugated with leptin. Specific radioactivity of ^125I-LEP was 1.43 MBq/μg, to- tal labelling rate of ^125I -37.80/40. The Bmax of ^125I-LEP-97.5% ,NSB-6.7 % and B0-43.7%. The sensitivity was 0.25μg/L , CV of within and between assay-7.8% and 12.9%, respectively. The recovery rate was 96. 4%-103.2%, there was no cross-reaction with TSH,LH,FSH, PRL,GH and Ins, the correlation coefficient between results of indirect iodination method and that of Linco Kit was 0. 951. Standard curve stability of ^125I-LEP prepared by indirect iodination was much better than that by the direct iodination in 45 days, which can satisfy the needs in clinic use.

关 键 词：消脂素 放射免疫分析 间接碘标记法 

分 类 号：R817[医药卫生—影像医学与核医学]