达纳康对类老年痴呆大鼠脑组织胶质细胞原纤维酸性蛋白和IL-1β、IL-6及APP mRNA表达的影响  

Influence of Danakang on GFAP,IL-1β and APP mRNA expressions in AD rats

作　　者：杨戈[1] 林水淼[2] 赵伟康[2] 徐品初[2] 周如倩[2] 郁志华[2] 

机构地区：[1]北京中国中医研究院广安门医院,北京100053 [2]上海市中医老年医学研究所,上海200032

出　　处：《北京中医药大学学报》2006年第12期816-819,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.39830450)

摘　　要：目的研究达纳康（EGb761）对β-淀粉样蛋白（Aβ）25—35所致类老年性痴呆（AD）模型大鼠神经免疫调节的作用机制。方法采用双侧杏仁核注射Aβ25—35造成类AD大鼠模型，观察大鼠莫里斯（Morris）水迷宫空间记忆能力，并分别通过免疫组化和RT—PCR方法研究类AD大鼠神经元胶质细胞原纤维酸性蛋白（GFAP）、APPmRNA表达；观察炎性细胞因子IL-1β、IL-6的变化以及EGb761的影响。结果类AD模型大鼠Morris水迷宫测试出现空间记忆能力下降，皮层、海马APPmRNA的表达上调；海马、皮层GFAP阳性胶质细胞和IL-6mRNA以及海马IL-1βmRNA表达增高。EGb761能够提高模型大鼠定向学习记忆能力，降低海马、皮层GFAP阳性胶质细胞表达，降低海马部位APPmRNA的表达。结论EGb761能显著改善AB诱导的痴呆模型大鼠学习记忆障碍，降低海马APPmRNA表达，减轻神经细胞的炎症和免疫联级反应。提示有效控制AD患者脑内AB免疫联级反应是EGb761的重要作用机制之一。Objective To study the mechanism of neuroimmunologic regulation of Danakang （EGb761） in rat model of Alzheimer＇ s disease （AD） induced by β-amyloid protein （Aβ）. Methods The AD rat model was established by injecting Aβ25 - 35 into rat bilateral amygdale. The spatial memory ability of AD model in Morris water maze （MWM） was observed, the expressions of glial fibrillary acidic protein （GFAP） and APPmRNA in AD rats were studied by using immunohistoch, emistry and RT-PCR, the changes of IL-1 β and IL-6, and the influence of EGb761 were observed. Result The spatial memory ability of AD model in MWM was decreased. The expressions of APPmRNA, GFAP, IL-6 mRNA and IL-1β in the cortex and hippocampus of AD model were increased. The result showed that EGb761 improved the oriental learning and memory ability, decreased the expressions of GFAP in hippocampus and cortex and APPmRNA expression of hippocampus in AD rats. Conclusion EGb761 can relieve significantly the disorder of learning and memory ability induced by Aβ in AD rat model, decrease APP mRNA expression of hippocampus, and alleviate the inflammation and immune reaction of neurons. One of the important mechanisms of EGb761 may be related to that it can control Aβ immune cascade reaction in the brain of AD patient.

关 键 词：达纳康 Β-淀粉样蛋白 胶质细胞原纤维酸性蛋白 白细胞介素-1 白细胞介素-6 β-淀粉样蛋白前体蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]