丹酚酸B对低血压小鼠脑缺血能量代谢的影响  

Influence of salvianolic acid B on energy metabolism of cerebral ischemia in mice with hypotension

作　　者：王秋华[1] 蒋玉凤[1] 汪芸[1] 王文荣[1] 张宇忠[1] 刘世静[1] 刘智勤[2] 黄启福[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]河北大学药学院,河北071002

出　　处：《北京中医药大学学报》2006年第12期820-822,825,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30472281)

摘　　要：目的观察丹酚酸B（SalB）对小鼠脑缺血能量代谢的影响。方法NIH小鼠分为假手术组、缺血组、SalB组和尼莫地平（Nim）组，测定脑组织线粒体膜电位（MMP）、脑能荷（EC）、磷酸肌酸（PCr）含量和Na^＋-K^＋-ATPase、Ca^2＋-ATPase的活性。结果脑缺血组脑缺血20、30min线粒体膜电位显著升高（P〈0．01），SalB组和Nim组可明显缓解脑缺血后线粒体膜电位的升高（P〈0．01）；脑缺血组脑组织EC、PCr的含量在缺血30min显著降低（P〈0．05～0．01），SalB组和Nim组可升高EC及PCr的含量（P〈0．01）；脑缺血组Na^＋-K^＋-ATPase和Ca^2＋-ATPase活性在脑缺血20、30min时显著下降（P〈0．01），而SalB组和Nim组可缓解Na^＋-K^＋ATPase、Ca^2＋-ATPase活性的下降（P〈0．05～0．01）。结论SalB改善脑缺血能量代谢可能与其调整不同的环节有关。Objective To observe the influence of salvianolic acid B （Sal B） on the energy metabolism of cerebral ischemia in mice. Methods The mice were randomly divided into the sham-operated group, cerebral ischemia group, Sal B group and Nimodipine （Nim） group. Brain tissue was collected to determine the mitochondria membrane potential （MMP）, energy charge （EC）, content of phosphocreatine （PCr）, activities of Na^＋ -K^＋ -ATPase and Ca^2＋ -ATPase. Results In cerebral ischemia group MMP was significantly increased after cerebral ischemia for 20 and 30 minutes （ P 〈 0.01 ）. The increase of MMP after cerebral ischemia was abated obviously in SalB group and Nim group （P 〈 0. 01 ）. In cerebral ischemia group the contents of EC and PCr of brain tissue were decreased remarkably 30 minutes after cerebral ischemia （P 〈 0. 05 - 0. 01 ）, while those were increased in SalB group and Nim group （ P 〈 0. 01 ）. The activities of Na ^＋ -K^＋ -ATPase and Ca^2＋ -ATPase in cerebral ischemia group were decreased evidently after cerebral ischemia for 20 and 30 minutes （ P 〈 0. 01 ）, but the decrease was markedly regulated in SalB group and Nim group （P 〈0.01 ）. Conclusion The mechanism that SalB can improve the energy metabolism of cerebral ischemia might be related to its different regulative effects.

关 键 词：丹酚酸B 脑缺血 能量代谢 小鼠 

分 类 号：R285.5[医药卫生—中药学]