抗稻瘟病菌株—诺卡氏菌A11的诱变育种  

Breeding of Norcardia sp.A11,a strain resistant to rice blast by induced mutation

作　　者：叶亚军[1] 张敏[1] 汤志良[1] 

机构地区：[1]四川农业大学植物保护系,雅安625014

出　　处：《植物保护》2007年第1期33-36,共4页Plant Protection

基　　金：四川省教育厅资助项目(2002A013)

摘　　要：从四川农业大学农场土壤中分离得到的诺卡氏菌(Norcardiasp.),其代谢产物对稻瘟病菌有强烈的抑制作用。以此菌株为出发菌株,进行紫外线(UV)、硫酸二乙酯(DES)、微波(MV)诱变,诱变处理后获得的突变株中以UV处理120 s时正变率最高,其摇瓶复筛试验中,该变异株代谢产物拮抗活性比出发株提高43.4%。MV处理在20 s剂量下提高了32.8%;DES处理在浓度为0.2%时提高了24.8%。经连续传代8次,拮抗活性稳定。A strain named A11 was isolated from the soil in farm fields in Sichuan Agricultural University. It was identified as Norcardia sp. through colony analysis and biochemical determination. It produced an antibiotic substance that could obviously inhibit Pyricularia Grisea. With All as a starting strain, some high-yield mutant strains were obtained after UV irradiation, DES treatment and microwave irradiafion. With UV irradiation for 120 s, the best forward-mutant strain was obtained. Through fermentation, the antibiotic activity of the mutant strain that was irradiated by UV for 120 s increased by 43. 4% compared with that of the starting strain. And the antibiotic activity of the mutant strain irradiated by MV for 20s increased by 32. 8% compared with that of the starting strain. Meanwhile, the antibiotic activity of the mutant strain treated with 0. 2% DES increased by 32. 8% compared with that of the starting strain. Their antibiotic activity was stable through 8 generations.

关 键 词：稻瘟病 诱变 诺卡氏菌 

分 类 号：S511.035[农业科学—作物学]