侵染紫松果菊的黄瓜花叶病毒分离物鉴定  

Identification of Cucumber mosaic virus isolated from Echinacea purpurea

作　　者：李桂芬[1] 朱水芳[1] 周群 施宗伟[1] 张永江[1] 李明福[1] 

机构地区：[1]中国检验检疫科学研究院动植物检验检疫研究所,北京100024 [2]山东省莱西市农业局植保站,266600

出　　处：《植物保护》2007年第1期54-56,共3页Plant Protection

摘　　要：从表现黄花叶症状的紫松果菊病株上获得分离物2-1-2,电镜下可见直径约30 nm的球状病毒粒体,其与黄瓜花叶病毒抗体呈强的阳性反应,ds-RNA的谱带类型与本实验室保存的标准黄瓜花叶病毒株系相同。通过生物学、病毒粒体观察、血清学以及病毒核酸双链试验结果,确定该病毒分离物为黄瓜花叶病毒。The virus particles of about 30nm in diameter were isolated from Echinacea purpurea Moench showing systemic sympotoms, including yellow mosaic on leaves. The virus-infected plants reacted positively with the Cucumber mosaic virus （CMV） antibody in triple antibody sandwich enzyme-linked immunosorbent assay （TAS- ELISA）. The type of the double-stranded RNA pattern of the isolate was the same as the standard CMV. On the basis of the biology, particle morphology, serology and ds-RNA pattern of the virus, it was concluded that the virus isolated from E. purpurea in Huairou, Beijing is Cucumber mosaic virus.

关 键 词：紫松果菊 黄瓜花叶病毒 鉴定 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]