转雪花莲凝集素酶基因枸杞对蚜虫的抑制作用  

Inhibition effects of the transgenic Lycium plants expressing snowdrop lectin on development of aphid population

作　　者：罗青 巫鹏举 李晓莺 贝盏临 曲玲 曹有龙 

机构地区：[1]宁夏枸杞发展工程技术研究中心,银川750002

出　　处：《植物保护》2007年第1期65-67,共3页Plant Protection

基　　金：国家科技部"九五"地方重大科技攻关项目(2000-104);宁夏自然科学基金(B1006)

摘　　要：利用农杆菌介导法将雪花莲外源凝集素酶基因导入枸杞叶片,获得了完整的转基因植株。对36个经PCR检测为阳性的转基因株系进行了两年抗蚜虫筛选试验,结果表明:蚜口密度抑制率在13.19%-95.4%,平均蚜口密度抑制率为78.59%。Agrobacterium-mediated transformation of Lycium was studied to prevent the pest aphid. The gene gna was successfully transferred into Lycium by using leaf dish, and a large number of completely transgenic plants were obtained. Thirty-six transgenic Lycium plants that had been confirmed as being gna-integrated by PCR detection were tested for resistance to aphids in two years. The results showed that the inhibition rate of aphid population was 13. 19%-95. 4%, 78. 59% on average.

关 键 词：枸杞 雪花莲外源凝集素酶基因 转化 蚜虫 抑制 

分 类 号：S47[农业科学—农业昆虫与害虫防治]