烟草环斑病毒DB-RT-Realtime PCR检测方法研究  

A study on detection of Tobacco ringspot virus by DB-RT-Realtime PCR

作　　者：郑耘[1] 杨伟东[1] 陈枝楠[1] 李明福 章桂明[1] 余道坚[1] 张永江 

机构地区：[1]深圳出入境检验检疫局,深圳518001 [2]中国检验检疫科学院动植物检疫研究所,北京100025

出　　处：《植物保护》2007年第1期117-120,共4页Plant Protection

基　　金：国家质量监督检验疫局资助项目(2002IK113)

摘　　要：本研究首次应用直接结合反转录实时荧光PCR技术(direct binding reverse transcription realtime PCR,DB-RT-Re-altime PCR)检测烟草环斑病毒,由于综合运用了PCR管吸附病毒外壳蛋白、病毒核酸分子杂交、高灵敏度实时荧光PCR技术的优点,从而使病毒检测在特异性、灵敏度、稳定性等技术指标上比传统的DAS-ELISA方法都有所提高,解决了烟草环斑病毒检测工作中由于隐症、干扰物质存在而影响检测结果的问题,为病毒检测提供了一个快速、简便有效的检测方法。Direct binding reverse transcription realtime PCR technique was used for detecting Tobacco ringspot virus. For the advantages of combining capsid protein-absorption by PCR tubes and molecular hybridization of virus nucleic acids with high sensitivity of Real-Time PCR, this technique is better in detection specificity, sensitivity and stability than the traditional DAS-ELISA method. It also resolved the problems such as symptom-masking and interfering materials in detecting Tobacco ringspot virus.

关 键 词：烟草环斑病毒 反转录PCR 检测 

分 类 号：S432.1[农业科学—植物病理学]