鸡肝脏谷胱甘肽转移酶的荧光光谱分析  

Fluorescence Spectra and Analysis of Chicken Liver GST

作　　者：薛泽春[1] 程新胜[1] 杨丽文[1] 张现[1] 席征[1] 

机构地区：[1]中国科学技术大学烟草与健康研究中心,安徽合肥230052

出　　处：《光谱学与光谱分析》2007年第1期99-103,共5页Spectroscopy and Spectral Analysis

基　　金：国家烟草专卖局科学基金项目(11020302016)资助

摘　　要：谷胱甘肽转移酶是生物体内一种重要的解毒酶,排除外源和内源毒素,维护肌体的正常功能。文章从荧光角度研究了谷胱甘肽转移酶光谱性质,得到谷胱甘肽转移酶的三维荧光谱图,确定了色氨酸、酪氨酸的荧光峰,以及肽链骨架荧光峰。根据其峰位的红移或蓝移,以及在不同pH条件下氨基酸荧光峰强度、位置变化,推断谷胱甘肽转移酶构象变化以及色氨酸在酶分子中的位置。Glutathione transferases （GST; EC2. 5.1.18） is an important detoxification enzyme which catalyze the conjugation of glutathione to a large variety of endogenous and exogenous electrophilic compounds to protect the functions of body. In the present paper, three dimensional fluorescence spectra were obtained, through which the authors could identify the fluorescence spectra of peptide bond, Tyr and Trp residue. The authors compared aromatic amino-acid residue fluorescence spectra in GST with dissociative to know red or blue shift of the fluorescence peak. The authors also studied the peptide bond and Trp residue fluorescence spectra at various pH, which suggested the change in GST surface and surface hydrophobicity, and the microenviroment change of aromatic amino-acid residue in enzyme.

关 键 词：谷胱甘肽转移酶 三维荧光 肽键 色氨酸 酪氨酸 

分 类 号：Q51[生物学—生物化学]