荧光法研究对羧基苯偶氮基杯[8]芳烃与诺氟沙星的作用  

Study on the Interaction between p-Octacarboxyphenylazocalix[8]arene and Norfloxacin by Fluorescence Spectrometry

作　　者：李艳平[1] 李来生[1] 黄志兵[1] 刘旭[1] 葛小辉[1] 

机构地区：[1]南昌大学分析测试中心,江西南昌330047

出　　处：《光谱学与光谱分析》2007年第1期108-112,共5页Spectroscopy and Spectral Analysis

基　　金：江西省教委基金项目(JJ2006-07);江西省自然科学基金项目(9920024)资助

摘　　要：合成了一种超分子探针对羧基苯偶氮基杯[8]芳烃(简称CPAC)。利用荧光光谱法研究了溶液状态下该探针与诺氟沙星(简称NFLX)的相互作用。实验表明,两者之间存在较强烈的相互作用,CPAC与NFLX形成外式包结物,静态猝灭NFLX的荧光,CPAC的杯腔体与NFLX的喹啉环间的疏水作用是主要作用方式。测定了该反应的结合常数(K=6.38×105L.mol-1)和结合比(n=1)。实验发现,小牛胸腺DNA能夺取CPAC-NFLX体系中的CPAC,使NFLX游离,说明超分子化合物CPAC可用于诺氟沙星药物的储存和定点释控。In the present paper, p-octacarboxyphenylazoealix[8]arene （CPAC） was used as supramolecular probe according to a reported method. The interaction of CPAC with drug norfloxacin （NFLX） was studied by fluorescence spectrometry. The results show that CPAC can strongly quench the fluorescence of NFLX because of the complex interaction between host and guest molecules in exo-inclusion complex. The spectral changes indicated that the quenching can be considered as static quenching mode. The hydrophobic interaction between the cavity of CPAC and the quinoline ring was the main force to consolidate the exo-inclusive complex CPAC-NFLX stability. The complex constant （K） and binding ratio （n） were determined to be 6. 38× 10^5 L ·mol^-1 and 1, respectively. Further experiment found that the calf thymus DNA and CPAC can combine, leading to the release of NFLX and the enhancement of fluorescence of the reaction system. It is expected that CPAC will be used as drug carrier and releaser.

关 键 词：荧光光谱法 超分子作用 芳烃 诺氟沙星 结合常数 

分 类 号：O657.3[理学—分析化学]