魟鱼软骨多糖对小鼠Lewis肺癌的作用及分子机制探讨  

Effect of Ray Cartilage Glycosaminoglycans on Lewis Lung carcinoma in C57BL/6J Mouse and Its Molecular Mechanism Exploration

作　　者：郭斌[1] 袁戈[2] 

机构地区：[1]锦州医学院附属第一医院,辽宁锦州121001 [2]锦州医学院附属第二医院,辽宁锦州121001

出　　处：《辽宁中医杂志》2007年第1期110-112,共3页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:研究魟鱼软骨多糖(RCG)对小鼠Lew is肺癌的作用,并初步探讨其作用机制。方法:建立小鼠Lew is肺癌模型,将实验分为生理盐水(Saline)组、不同剂量魟鱼软骨多糖(RCG)组、环磷酰胺(CTX)组,计算原发瘤抑制率及肺转移数;采用RT-PCR和W estern b lot检测原发瘤组织VEGF、bFGF mRNA和蛋白表达。结果:应用魟鱼软骨多糖各剂量组,小鼠原发瘤抑制率、肺转移数与生理盐水组比较差异有显著性(P<0.05,P<0.01);与生理盐水比较,魟鱼软骨多糖各剂量组,VEGF、bFGF mRNA和蛋白表达水平显著降低(P<0.01)。结论:魟鱼软骨多糖明显抑制小鼠Lew is肺癌原发瘤的生长和转移,可能与其抑制肿瘤VEGF、bFGF mRNA和蛋白表达有关。Objective： To investigate effect of Ray Cartilage Glycosaminoglycans （RCG）on Lewis lung carcinoma in C57BL/ 6J mouse and to explore its molecurlar mechardsm. Methods： The model of mouse with Lewis lung carcinoma was made, the experimental mice were randomly divided into normal saline group, RCG groups at varied concentrations and CTX group, tumor in- hibitory rate and number of lung metastasis focuses were measured;the mRNA and protein expression of VEGF, bFGF were deter- mined with RT - PCR and Western blot. Results ： There were significant differences from inhibitory rates of primary tumor and the number of lung metastasis focuses between RCG groups and Saline group（ P 〈0.05, P 〈0.01） ;VEGF,bFGF mRNA and protein expression levels in RCG groups were lower significantly than in Saline group. Conclusions： RCG could effectively inhibit the growth and metastasis of implanted Lewis lung carcinoma in C57BL/6.I mouse , which was probably the reason that RCG reduced the expressions of VEGF,bFGF mRNA and protein of Lewis lung carcinoma.

关 键 词：魟鱼软骨多糖 LEWIS肺癌 VEGF BFGF 

分 类 号：R286.91[医药卫生—中药学] R979.1[医药卫生—中医学]