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作 者:张志勇[1] 王刚卫[1] 田晓莉[1] 段留生[1] 王保民[1] 董学会[1] 张明才[1] 何钟佩[1] 李召虎[1]
机构地区:[1]中国农业大学农学与生物技术学院,北京100094
出 处:《棉花学报》2007年第1期47-51,共5页Cotton Science
基 金:国家自然科学基金(30100111)
摘 要:在水培条件下研究了转基因抗虫棉中棉所41和常规棉中棉所36、中棉所35三个品种苗期钾吸收效率的差异及其机制。结果表明,在营养液中钾浓度为0.5mmol·L^-1,可使棉花幼苗生长速率达到最高生长速率80%左右的条件下,中棉所35和中棉所41在6~7叶期的生物量和体内钾吸收量显著高于中棉所36。中棉所35的吸钾量多主要与其根系活跃吸收表面积大有关;中棉所41的吸钾量多可能是相对较大的根系活跃吸收表面积和相对较高的Ⅰmax综合作用的结果。中棉所36的Ⅰmax虽然显著高于其它两个品种,但体内钾累积量却比较低,这与其较小的根系活跃吸收表面积有关,该品种体内较高的钾浓度也可能对吸收产生反馈抑制作用。在本试验条件下,转基因抗虫棉中棉所41的苗期钾吸收能力与常规棉相比并不低。In order to identify whether susceptibility of cotton to K deficiency is related with Bt gene and/or CpTI gene introduced into cotton, this work, under hydroponic culture, explored the differences in K uptaking among transgenic Bt and CpTI cotton cultivars ( CCRI 41) and conventional cotton cultivars ( CCRI 36 and CCRI 35). The results showed that seedlings of CCRI 35, CCRI 41 at 6- 7 leaf stage had significantly higher biomass and K accumulation than CCRI 36, while cultivated at 0.5 mmol·L^-1 K, which enable about 80% of the most growth rate of cotton seedlings. More K accumulation in CCRI 35 plant was mainly due to its significantly higher active absorbing surface area of root (AASA). However, comparatively higher AASA and comparatively higher Ⅰmax maybe caused more K accumulation in CCRI 41 plant comprehensively. Despite of its highest Ⅰmax among three eultivars, K accumulation in CCRI 36 plant was less , which might be attributed to its significantly lower AASA and feedback inhibition on K uptaking by higher K concentration in the plant. Under the condition of this experiment, there was significant difference in K accumulation among cotton eultivars, but which was not necessarily pertinent to Bt-gene and CpTI gene introduction.
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