柱前衍生高效液相色谱法测定视明注射液中麝香酮含量  

HPLC determination of muscone in Shiming injection with precolumn derivatization

作　　者：杨蕾[1] 包小红[2] 黄瑛[2] 刘仲义[2] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川省食品药品检验所,成都610041

出　　处：《药物分析杂志》2007年第1期60-62,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立将2,4-二硝基苯肼与视明注射液进行柱前衍生化反应,以 HPLC-UV 测定的分析方法。方法:使用江苏淮阴精化 C_(18)(4.6 mm×200 mm,5μm)色谱柱;乙腈-水(90∶10)为流动相,流速:1.0 mL·min^(-1);检测波长为365 nm。结果:麝香酮在1.60～7.00μg范围内线性关系良好,r=0.9997;平均回收率(n=3)为97.5%,RSD=1.6%。结论:该方法专属性强,灵敏准确,重现性好,为视明注射液的质量控制提供了有效的检测手段。Objective：To develop an HPLC - UV analysis method for determining muscone hydrazone of Shiming injection with2,4- dinitrophenylhydrazine derivative. Method：Using C19 column（4. 6 mm × 200 mm, 5μm）, acetonitrile -water（90： 10）as mobile phase ;The flow rate was 1.0 mL · min^-1 and the detection wavelength was 365 nm. Results： The calibration curves showed good linearity over the range of 1.60 - 7. 00μg, r = 0. 9997 ; the average recovery was 97.5%, RSD = 1.6%. Conclusion：The method is specific, sensitive, accurate and suitable for the determination of muscone in Shiming injection.

关 键 词：麝香酮 固相萃取 2 4-二硝基苯肼 柱前衍生化 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]