氧氟沙星的测定及其与DNA相互作用的研究  

Determination of ofloxacin and its interaction with DNA

作　　者：漆红兰[1] 陈沛[1] 张成孝[1] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062

出　　处：《药物分析杂志》2007年第1期96-99,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(20375025)

摘　　要：目的:建立测定氧氟沙星的分析方法,研究氧氟沙星与 DNA 的相互作用,初步探讨氧氟沙星与 DNA 相互作用的机理。方法:在0.20 mol·L^(-1)醋酸-醋酸钠(pH=5.0)中,研究氧氟沙星在碳纳米管糊电极上的电化学行为,建立电化学测定氧氟沙星的分析方法。利用电化学法和荧光法研究氧氟沙星与 DNA 的相互作用。结果:在碳纳米管糊电极上,氧氟沙星的检出限为8.5×10^(-8)mol·L^(-1)。氧氟沙星与 DNA 发生插入作用。结论:建立了灵敏测定氧氟沙星的电化学分析方法,利用电化学方法可研究氧氟沙星与 DNA 的相互作用。Objective： To develop the electrochemical method for the determination of ofloxacin （OFLX） and to investigate the interaction between ofloxacin and DNA. Methods： In 0. 20 mol · L^-1 acetate buffer solution （ pH = 5.0）, the electrochemical behaviors of ofloxacin at carbon nanotube paste electrode were studied by cyclic voltammetry to develop an electrochemical method for the determination of ofloxacin. The interaction of ofloxacin with DNA was studied by electrochemical and fluorescence methods. Results ：The detection limit of ofloxacin was 8.5×10^-8mol·L^-1. Ofloxacin could bind with DNA by intercalation. Conclusion： An electrochemical method for the determination of ofloxacin is developed with high sensitivity. The interaction of ofloxacin and DNA can be investigated by electrochemical method.

关 键 词：氧氟沙星 DNA 电化学 荧光 

分 类 号：R917[医药卫生—药物分析学]