内蒙古白刺属植物遗传多样性的RAPD分析  

RAPD analysis on genetic diversity of nitavia in inner mongolia

作　　者：宛涛[1] 蔡萍 伊卫东[1] 张晓明[3] 张辰波[1] 

机构地区：[1]内蒙古农业大学生物工程学院,内蒙古呼和浩特010018 [2]锡林浩特牧业学院,内蒙古锡林浩特026000 [3]呼伦贝尔大学,内蒙古海拉尔021008

出　　处：《干旱地区农业研究》2007年第1期225-229,共5页Agricultural Research in the Arid Areas

基　　金：国家自然科学研究基金项目(30360070)

摘　　要：利用随机扩增DNA多态性（RAPD）技术对4种不同地理种群的白刺属（Nitraria L．）植物进行了遗传多样性分析。15个引物扩增出118个位点，其中多态性位点95个（80．5％），各引物的Nei＇s指数和Shannon＇s多样性指教差别较大，利用UPGMA方法构建分子系统树。结果表明：白刺属植物的遗传分化程度和基因流因种而异，除球果白刺以外，其余3种白刺的种群间有很大的基因流，其遗传一致度在0．8561～0．9694之间，而种间遗传分化程度较低，故4种白刺有着相近的祖先，这与形态学和细胞学的分析结果一致，说明RAPD分析方法可从分子生物学角度为白刺属植物的系统学分类提供更为有利和可靠的证据。Four species of Nitraria were analyzed using random amplified polymorphic DNA （RAPD） markers. 15 random primers amplified 118 loci have 95 polymorphic loci, about 80.5%. The differentees of Nei＇s gene diversity and Shannon ＇ s information index between each primers produced is big. Dendrogram was constructed using UPGMA. The result showed that the polarization rof populations and gene fluxes are different in different species. The polarization and gene fluxes of populations of 3 Nitavias are lower except N. sphaerocarpa . The heredity coherence of each population is change from 0. 8561 to 0. 9694. The polarization in the populations is low, and they have the together ancestor. This is similar to morphology and cytology result, so RAPD is a useful supple mentary method in assessing genetic relationship among Nitraria species.

关 键 词：白刺属 RAPD分析 亲缘关系 遗传变异 

分 类 号：S793.9[农业科学—林木遗传育种]