利福霉素B发酵过程中巴比妥钠加量的优化  

Optimization of Barbital Sodium Content during Fermentation of Rifamycin B

作　　者：王军峰 杜吉泉 

机构地区：[1]沈阳同联制药集团上海研究所,上海200237 [2]济南高新技术开发区管委会,山东济南250101

出　　处：《食品与药品》2007年第02A期11-13,共3页Food and Drug

摘　　要：目的优化利福霉素发酵摇瓶中巴比妥钠的添加量及添加策略,以提高发酵产量。方法采用摇瓶发酵,优化巴比妥钠初始含量及中间补加策略,通过测定发酵液中利福霉素B的效价及菌体浓度来确定合适的发酵工艺。结果巴比妥钠添加的优化策略为:初始浓度0.05%,72h补加至0.1%,120h补加至0.15%。结论优化工艺使发酵单位显著提高,比不加巴比妥钠提高17%,比目前生产水平提高10%以上。Objective To optimize the content and feeding strategy of barbital sodium during the fermentation of rifamycin B in order to improve the yield. Methods In shaken flask culture, the content and feeding strategy of barbital sodium during the fermentation of rifamycin B was optimized and the suitable fermentation technology was also defined by determining the potency of rifamycin B and PMV of mycelia in fermentation broth. Results The optimal feeding strategy of barbital sodium was obtained, which was that the incipient concentration of barbital sodium was 0.05% in base medium and was added to 0.1% at 72 h, 0.15% at 120 h. Conclusion The yield of rifamycin B is raised significantly with the technology optimized. The productivity of rifamycin B is increased by 17% than that without barbital sodium and 10% more than that produced at the present level.

关 键 词：利福霉素 地中海拟无枝酸菌 巴比妥 发酵优化 

分 类 号：R978.15[医药卫生—药品]