草地藏系绵羊心脏脂肪酸结合蛋白基因第二内含子的克隆和测序  

Clone and Sequence of the Second Intron of Heart Fatty Acid Binding Protein Gene of Tibetan Sheep in Pasture

作　　者：梁梓[1] 郑玉才[1] 王永[1] 傅昌秀[2] 金素钰[1] 王金玲[1] 於建国[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省畜禽繁育改良总站,四川成都610041

出　　处：《草业与畜牧》2007年第2期44-47,共4页Pruataculture & Animal Husbandry

基　　金：四川省十五攻关项目"草地藏系绵羊的选育与提高"(02NG004-056);国家民委重点项目"草地藏系绵羊肉用性能的改良与利用"(03N010)的一部分

摘　　要：采用PCR技术对草地藏系绵羊心脏脂肪酸结合蛋白基因第2内含子进行了扩增,扩增产物经克隆和测序显示,其长度为1 893bp,该基因片段与GenBank中普通绵羊和猪的H-FABP基因第2内含子分别有99%和66.4%的同源性。实验还对22只草地藏系绵羊H-FABP基因第2内含子中174 bp的序列进行了PCR-SSCP分析,结果未检测到多态性。The second intron of heart fatty acid binding protein （H-FABP） gene of Tibetan sheep in Pasture was amplified by PCR method. The amplified PCR product was cloned and sequenced. The results showed that the length of fragment of H-FABP was 1 893 bp and this fragment had 99% and 66.4% of homology with the second intron of H-FABP gene of common sheep and porcine in GenBbank respectively.In addition, A 174bp-fragment of the second intron of H-FABP gene was assayed in 22 DNA samples from Tibetan sheep by PCR-SSCP, but no oolvmorohism was observed.

关 键 词：草地藏系绵羊 心脏脂肪酸结合蛋白 单链构象多态性 

分 类 号：S826[农业科学—畜牧学] Q785[农业科学—畜牧兽医]