固定化白腐菌产漆酶培养条件的研究  

Study on the optimum culture conditions of production laccase by immobilized white rot fungi

作　　者：朱雄伟[1] 刘玉兰[2] 胡道伟[3] 

机构地区：[1]武汉工程大学化工与制药学院湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430074 [2]武汉工程大学材料科学与工程学院,湖北武汉430074 [3]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《武汉工程大学学报》2007年第1期13-15,共3页Journal of Wuhan Institute of Technology

摘　　要：采用三种固定杂色云芝菌的方法,对杂色云芝菌在固定化的培养条件下的产漆酶条件进行了研究.实验结果表明:漆酶产量高的碳源是葡萄糖,氮源是氯化铵,pH值为3.6,表面活性剂、诱导剂对漆酶的活性影响不大;漆酶产量高的是海藻酸钙-聚乙烯醇双载体固定化法,载体系统内的酶活比系统外的高.In the paper,the experiments adapted three methods of immobilized white rot fungi to study the optimum culture conditions were carried out. The experimental results show that the optimum culture conditions are as follows：the source of carbon, the source of azores and pH value are glucose, NH4Cl and 3.6, respectively. And the yield of iaccase is lower than in the self - immobilization, the revuslant and the surface alive have no effect on the production of laccase in the condition of immobileization.

关 键 词：杂色云芝菌 固定化 漆酶 

分 类 号：Q939.9[生物学—微生物学]