怀山药多糖对鸡免疫功能的影响  

Effects of Dioscorea opposita polysaccharide on immune function in chickens

作　　者：张红英[1,2] 崔保安[1,2] 邱妍[3] 王远阁[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南省动物性食品安全重点实验室 [3]南京农业大学动物医学院

出　　处：《中兽医医药杂志》2007年第1期11-13,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家"十五"食品安全重大攻关专项(2001BA804A30-11);河南省杰出人才创新基金(0621002100)

摘　　要：目的研究怀山药多糖对鸡免疫功能的影响。方法将150羽14日龄蛋公鸡随机分成3组,每组50羽,14日龄用一羽份新支二联弱毒苗点眼滴鼻免疫;同时2个试验组分别肌肉注射怀山药多糖溶液高、低剂量各0.3ml/羽,对照组注射生理盐水0.3ml/羽,连用3d;分别于免疫用药后的第7、14、21、28、35、42天各组随机抽取8只翼下静脉采血,后分离血清,测定新城疫血凝抑制(HI)抗体效价,并于免疫后14、21、28、35、42天随机抽4只放血致死后称重,摘取胸腺、法氏囊、脾脏称重并计算指数。结果试验组特异性免疫抗体和免疫器官指数均高于对照组,在某些时间点差异显著。结论怀山药多糖能增强鸡的免疫功能,可作为免疫增强剂与疫苗同时使用。Objective： To study the effects of Dioscorea opposita polysaccharide （DOPS） on immune function in chickens. Methods： 150 14-day- old chickens were randomly assigned to 3 groups at 50 each. All groups were vaccinated with ND -IBD vaccine on 14^th ; meanwhile, high and low dose of DOPS were given by muscular injection at 0. 3 ml each in 2 experimental groups, and the control were given physiological saline for 3 days. On 7^th, 14^th, 21^th, 28^th , 35^th and 42^nd after vaccination, 8 blood samples were randomly obtained from each group for the determination of the level of HI antibody against ND. On 14^th, 21^st , 28^th , 35^th and 42^nd after vaccination, 4 chickens＇whole body, spleen, bursa of Fabrieius and thymus were randomly weighted from each group for the calculation of immune organ index. Results： The titer of special antibody and the immune organ indices of experimental groups were higher than that of the control, and the difference was significant at certain time points. Conclusion： DOPS coinoculated with ND vaccine can enhance immune responses in chickens.

关 键 词：怀山药多糖 新城疫疫苗 抗体 免疫器官指数 

分 类 号：S852.4[农业科学—基础兽医学]