树突状细胞前体细胞亚群四色荧光分析方法探讨  

Analysis of predendritic cell subsets in cord blood,peripheral blood and bone marrow

作　　者：李玉珠[1] 史敦云[1] 张琼丽[1] 

机构地区：[1]深圳市第二人民医院,518039

出　　处：《山西医药杂志》2007年第1期5-7,共3页Shanxi Medical Journal

基　　金：深圳市卫生科技计划项目(200204052)

摘　　要：目的建立树突状细胞(DC)前体细胞(pDC)亚群四色荧光检测方法,探讨以Lin-人类白细胞抗原(HLA)-DR+细胞群和以CD34-Lin-HLA-DR+细胞群设门检测不同来源标本pDC亚群的适用性。方法以Lin-HLA-DR+细胞群和以CD34-Lin-HLA-DR+细胞群设门分别检测健康成人外周血、脐带血、骨髓、粒细胞集落刺激因子(G-CSF)动员外周血pDC亚群各15例,比较两种设门方法的pDC检测结果。结果以该两种方法设门,pDC1/pDC2比值:外周血=脐带血>骨髓>G-CSF动员外周血;对同一种标本,两种设门方法之间比较,pDC1/pDC2比值差异无统计学意义;外周血CD34-Lin-HLA-DR+/单个核细胞(MNC)与Lin-HLA-DR+/MNC比值、pDC/CD34-Lin-HLA-DR+比值与pDC/Lin-HLA-DR+比值差异无统计学意义;而脐带血、骨髓、G-CSF动员外周血的CD34-Lin-HLA-DR+/MNC比值均显著性低于Lin-HLA-DR+/MNC比值,pDC/CD34-Lin-HLA-DR+比值显著性高于pDC/Lin-HLA-DR+比值。结论检测pDC亚群,对一般外周血标本,以Lin-HLA-DR+细胞群设门或以CD34-Lin-HLA-DR+细胞群设门,均可取得理想结果;对CD34+细胞含量相对丰富的脐带血、骨髓、G-CSF动员外周血等的标本,以CD34-Lin-HLA-DR+细胞群设门能明显优化检测结果。Objective To explore a suitable method to detect predendritic cell （pDC） subsets in cord blood, peripheral blood, bone marrow and G-CSF-mobilized blood. Methods pDC subsets in cord blood, peripheral blood, bone marrow and G-CSF-mobilized blood were detected gated by Lin^-HLA-DR^＋ cell population or CD34^-Lin^-HLA-DR^＋ cell population, pDC1/pDC2, CD34^-Lin^-HLA-DR^＋ cell/mononuclear cell （MNC） and Lin^-HLA-DR^＋ cell/MNC, pDC/CD34^-Lin^-HLA-DR^＋ cell and pDC/Lin^-HLA-DR^＋ cell were compared between two gating methods. Results pDC1/pDC2 ratio in cord blood, peripheral blood, bone marrow and G-CSF-mobilized blood, CD34^-Lin^-HLA-DR^＋ cell/MNC ratio and Lin^-HLA-DR^＋ cell/MNC ratio, pDC/CD34^-Lin^-HLA-DR^＋ cell ratio and pDC/Lin^-HLA-DR cell ratio in peripheral blood were not remarkably distinct between two gating methods （P〉0.05）, but detecting CD34^-Lin^-HLA-DR^＋ cell/MNC were lower than Lin^-HLA-DR^＋ cell/MNC （P〈0.05）, pDC/CD34^-Lina-HLA-DR^＋ cell were higher than pDC/Lin^-HLA-DR^＋ cell in cord blood, bone marrow and G-CSF-mobilized blood （P 〈 0.05）. Conclusion pDC subsets in cord blood, bone marrow and G-CSF mobilized blood should be analyzed by gating by CD34^-Lin^-HLA-DR^＋ cell population, and pDC subsets in peripheral blood can be analyzed by gating by Lin^-HLA-DR^＋ cell population or CD34^-Lin^-HLA-DR^＋ cell population beetle enriching CD34^＋ cells gated by Lin^-HLA-DR^＋ cell population were very fewer than in cord blood, bone marrow and G-CSF-mobilized blood.

关 键 词：树突细胞 粒细胞集落刺激因子 抗原 CD11c 

分 类 号：R392.1[医药卫生—免疫学]