重组人酸性成纤维细胞生长因子对糖尿病大鼠难愈性皮肤溃疡的促愈合作用  

Effects of recombinant human acidic fibroblast growth factor on healing of dermal chronic ulcers in diabetic rats

作　　者：刘青[1] 郭蔚莹[1] 刘克辉[1] 谢晓娜[1] 王丽颖[2] LIU Qing;GUO Wei—ying;LIU Ke-hui;XIE Xiao—na;WANG Li-ying(Department of Endocrinology,First Hospital,Jilin University,Changchun 130021,China;,Department of Molecular Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第一医院内分泌科,吉林长春130021 [2]吉林大学基础医学院分子生物学教研室,吉林长春130021

出　　处：《吉林大学学报(医学版)》2007年第1期61-63,共3页Journal of Jilin University:Medicine Edition

基　　金：国家973重大基础科研基金资助课题(2001CB510000)

摘　　要：目的:研究外用大肠杆菌表达的重组人酸性成纤维细胞生长因子(rhaFGF)对糖尿病大鼠难愈性创面的促愈合作用。方法:雄性Wistar大鼠10只,制备糖尿病大鼠难愈性皮肤溃疡模型;创面局部分别用rhaFGF(90U.cm^(-2))及生理盐水喷洒;记录创面面积、伤腔容积及愈合时间,观察创面肉芽组织生长及再上皮化,评价创伤修复情况。结果:与生理盐水对照组比较,rhaFGF组伤后各时间点的创面面积、伤腔容积均明显缩小(P<0.05),愈合率显著提高(P<0.05),创面愈合时间明显缩短(P<0.05)。rhaFGF组的肉芽组织生长及再上皮化明显加速。结论:局部应用大肠杆菌表达的rhaFGF对糖尿病大鼠难愈性创面有明显的促愈合作用。Objective To study the healing effect of recombinant human acidic fibroblast growth factor(rhaFGF)expressed in E.coli on dermal chronic ulcers in diabetic rats.Methods Ten male Wistar rats were used to set up diabetic dermal chronic ulcers models.The wounds were sprinkled with rhaFGF and physiological saline,respectively.The wound area,wound cavity volume and healing time were recorded,granulation tissue growth and epithelization in wound were observed,and wound healing status was evaluated.Results Compared with control group,the wound area and wound volume at different day in rhaFGF group were significantly diminuted(P〈0.05),the healing rate was predominantly elevated(P〈0.05),the healing time was significantly shortened(P〈0.05).RhaFGP resulted in a remarkable enhancement of granulation tissue growth and epithelization in wound.Conclusion The topical application of rhaFGF can significantly accelerate the healing of dermal chronic ulcers in diabetic rats.

关 键 词：成纤维细胞生长因子/药理学 疾病模型 动物 糖尿病 刨面愈合 

分 类 号：R587.2[医药卫生—内分泌] R-332[医药卫生—内科学]