TrkA在胚胎期Wistar大鼠端脑内的表达  

Expression of TrkA in Wistar rat brain during embryonic phase

作　　者：王晓明[1] 陈东[1] 

机构地区：[1]广东医学院组织学与胚胎学教研室,广东湛江524023

出　　处：《吉林大学学报（医学版）》2007年第1期81-83,F0003,共4页Journal of Jilin University：Medicine Edition

基　　金：教育部博士点专项基金资助课题(20030183048)

摘　　要：目的：探讨TrkA受体在胚胎不同时期Wistar大鼠端脑内的表达情况。方法：成年Wistar大鼠合笼饲养，查到阴栓或阴道涂片查到精子为EO天，分别在E13、E15、E17、E19和E21天利用常规形态学手段免疫组织化学方法观察胚胎期Wistar大鼠不同脑区TrkA表达情况。结果：E13天胎脑组织内未检测到TrkA阳性表达，E15天TrkA表达阳性率在胚胎期大鼠端脑皮质内呈上升趋势，E17天达次高峰，E19开始下降，出生前E21天再次达表达高峰。胎鼠纹状体、海马的发育较晚，E17天开始发育，TrkA在其内的表达与皮质内相似。结论：TrkA在胎鼠端脑的表达始于E15天，且自E15～E21表达量呈持续增加趋势，符合胚胎诱导过程。Objective To detect the expression of TrkA in the brain of the Wistar rats during the embryonic phase. Methods Adult Wistar rats were fed in one cage. It was E0 day when sepermia were found in vaginal suppository and vaginal smear. The expressions of TrkA in the brain of Wistar rats during various brain regions were observed by immunohistochemistryon E13, El5, El7, El9 and E21 day. Results On E13 day no obvious expression of TrkA was observed in the embryonic brain. The expression of TrkA had an obviously increasing tendency in the cortex of telencephalon from E15 day to E17 day. And on E17 day, the positive rate of the expression of TrkA reached the second peak. While on E19 day, the expression of TrkA had a temporary decreasing. Then on E21 day the expression of TrkA reached the highest peak. The striatum and hippocampus developed later than the telencephalon till El7 day, and the expressions of TrkA during these stages were same as the cerebrum. Conclusion From E15 to E17 day, obvious expressions of TrkA are observed in the embryonic telencephalon, and it accords with embryonic induction.

关 键 词：免疫组织化学 受体 神经生长因子 大鼠 Wistar胚胎诱导 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学] R-332[医药卫生—基础医学]