ECLIA法检测血N端脑钠尿肽水平与维持性血透析治疗病人的关系  

The Relationship Between Detect ion NT-proBNP by Electrochemiluminescene Immunoassay and Maintenance Hemodi- alysis Patients

作　　者：李美珠[1] 温少磊[1] 杨长兰[1] 林爱珍[1] 

机构地区：[1]佛山市第一人民医院检验科,广东佛山528000

出　　处：《国际医药卫生导报》2007年第2期86-88,共3页International Medicine and Health Guidance News

摘　　要：目的探讨应用电化学发光免疫分析技术（ECLIA）检测血N端脑钠尿肽（NT-proBNP）水平在慢性肾功衰竭、血液透析（HD）患者中的变化规律及临床意义。方法应用电化学发光免疫分析技术（ECLIA）测定143例慢性肾衰患者在血液透析前、后的血NT-proBNP水平，并以日立7170A生化分析仪测定总胆固醇（TCH）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、尿素氮（BNU）、肌酐（CRE）、和白蛋白（ALB），然后与60名健康者对照比较。结果患者NT-proBNP平均水平分别为：慢性肾功能衰竭（CRF）透析前组（2708．9±1609）ng／L，血液透析后组（1304．9±士1509．5）ng／L，均明显高于对照组（88．27±22．5）ng／L，三者之间的差异具有显著性（P〈0．01）慢性肾衰透析前组NT-proBNP水平变化与肌酐水平有正相关关系（r=0．63，P〈0．05），与ALB呈负相关关系（r=-0．72，P〈0．01）在血脂分析中，慢性肾衰血液透析前、后组与正常对照组有显著性差异，（P〈0．01），除了TG在血液透析后组与正常对照组无显著性差异。结论慢性肾衰患者血液透析前后血N端脑钠尿肽（NT-proBNP）水平郝升高，NT-proBNP检测对慢性肾功能衰竭患者心血管并发症的监测具有敏感性高，无创伤性的优点，在预防和治疗方面有一定的临床意义。Objective Discuss the variety and clinical meaning of NT-proBNP determination by electrochemiluminescent i,,nunoassay（ECLIA） method in chronic renal failure and hemodialysis patients. Method Test the NT-proBNP of 143 samples from chronic renal failure patient before and after hemodialysis by ECLIA method, and test the TCH, TG, HDL-C, LDL-C, BUN, CRE and ALB by Hitachi 7170A, then compare the results with 60 samples from the healthy persons. Result The average level of NT- proBNP for different tests： chronic renal failure patient befor hemodialysis is （2708.9 ± 1609） ng/ L, after hemodialysis is （1304.9 ± 1509.5） ng/L, both are obviously higher than the control group which is （88.27±22.5） ng/L, there is significant difference （P〈0. 01）;the NT-proBNP level of chronic renal failure patient before hemodialysis has positive relationship with CRE （r=0.63 and P 〈0.05）, but has negative relationship with ALB（r=-0. 72and P〈0. 01）, in the lipid analysis, the chronic renal failure before hemodialysis group has distinguish difference from the control group, but after hemodialysis group only TG hasn＇t. Conclusion The NT-proBNP level of chronic renal failure patient both is high before or after hemodialysis ,the detection of NT-proBNP is high sensitive and no traumatic for chronic renal failure together with cardiovascular complication, there is some clinical value in prevention and treasure.

关 键 词：B-型钠尿肽 慢性肾功能衰竭 血液透析 N端脑钠肽 

分 类 号：R692[医药卫生—泌尿科学]