荧光定量聚合酶链反应检测乙型肝炎患者血清HBV DNA及临床价值  

Detection of serum HBV DNA in patients with hepatitis B using quantitative fluorogenic probe polymerase chain reaction

作　　者：范公忍[1] 屠玉玲[1] 李娟[1] 闻炜[1] 邓涛[1] 任永强[1] 

机构地区：[1]北京军区总医院解放军肝病研究所,北京100700

出　　处：《实用肝脏病杂志》2007年第1期16-18,共3页Journal of Practical Hepatology

摘　　要：目的探讨荧光定量聚合酶链反应法定量检测乙型肝炎病毒(HBV)核酸的临床应用价值。方法应用荧光定量聚合酶链反应定量检测1962例乙型肝炎患者血清HBV DNA水平并与血清HBV标志物进行比较,并对35例乙型肝炎患者在拉米夫定治疗前后血清HBV DNA含量及相关指标作动态观察。结果乙型肝炎患者HBVDNA定量范围在6.79×102～1.95×109拷贝/毫升之间,在HBeAg阳性患者,其检出率和定量拷贝数较高。拉米夫定治疗后患者外周血HBV DNA载量下降明显。结论荧光定量PCR法是一种相对准确、灵敏度高、特异性强的定量检测HBV DNA的技术,对乙型肝炎诊断、指导、临床用药和疗效监测方面具有实用价值。Objective To detect serum HBV DNA in patients with hepatitis B by quantitative fluorogenic-probe polymerase chain reaction. Methods Serum HBV DNA in 1992 patients with hepatitis B was detected by using quantitative flurogenic-probe polymerase chain reaction. Results The serum HBV DNA level in patients with hepatitis B ranged from 6.79 × 10^2 to 1.95 ×10^9 copies/ml. After lamivudine treatment, HBV DNA load in the peripheral blood significantly decreased. Conclusion Quantitative fluorogenic-probe polymerase chain reaction is an accurate, highly sensitive, specific and simple technique for detection of serum HBV DNA.

关 键 词：慢性乙型肝炎 乙型肝炎病毒核酸 定量聚合酶链反应 拉米夫定 

分 类 号：R450[医药卫生—治疗学]