表皮葡萄球菌医院感染耐药性的基因型研究  

Molecular Mechanism of Antibiotic Resistance in Hospital Acquired Staphylococcus epidermidis Infection

作　　者：朱学源 刘诗强[2] 陈旭[2] 王微[1] 许浩东[1] 倪语星[2] 

机构地区：[1]上海市第八人民医院,上海200235 [2]上海交通大学瑞金医院,上海200020

出　　处：《中华医院感染学杂志》2007年第1期1-3,共3页Chinese Journal of Nosocomiology

基　　金：上海市自然科学基金项目(03ZR14074)

摘　　要：目的研究医院感染表皮葡萄球菌(SEP)耐药的分子机制,以控制其医院感染的发生。方法对分离的18株表皮葡萄球菌进行耐药分析,抽提细菌染色体DNA,用聚合酶链反应(PCR)扩增mecA、gyrA和grlA基因,用酶切、测序检测这些基因的存在及变异,分析与耐药的关系。结果18株表皮葡萄球菌中对甲氧西林耐药15株,耐药率83.3%,耐药菌株全部检测到mecA基因;18株中对喹诺酮类(诺氟沙星和环丙沙星)、克拉霉素、红霉素和克林霉素均耐药者11株,其中10株存在gyrA和(或)grlA突变;gyrA突变点为gyrA 84位TCA→TTA,grlA突变点为grlA80位TCC→TTC。结论医院感染表皮葡萄球菌存在严重的多重耐药现象,必须进一步规范临床用药,以减少耐药性的增加。OBJECTIVE To study the molecular mechanism of antibiotic resistance in hospital acquired Staphylococcus epidermidis infection, so as to reduce the occurrence of drug resistance and infection acquired in hospital. METHODS DNA from 18 strains of S. epidermidis were extracted by the phenol-chloroform method, and mecA, gyrA and grlA were amplified by PCR, then the variation of gyrA and grlA was tested by Hinf I- digested analysis. RESULTS Fifteen from 18 S. epidermidis strains were resistant to meticillin, and all of them had mecA gene. Eleven from 18 S. epidermidis strains were resistant to meticillin, quinolones and other antibiotics. And they all had a mutant in gyrA and/or grlA. The mutated spots were gyrA Ser84 （TCA→TTA） and GrlA Ser80（TCC→TTC）. CONCLUSIONS The majority of hospital acquired S. epidermidis strains are multiresistant to meticillin, quinolones and other antibiotics, which are caused by acquirement of drug-resistance gene or mutation of drug-targeting genes. Medical institutions must strictly standardize the application of antibiotics to reduce development of drug resistance.

关 键 词：表皮葡萄球菌 医院感染 MECA基因 

分 类 号：R378.11[医药卫生—病原生物学]