小麦×玉米单倍体植株再生体系的建立  

Establishment of Regeneration System in Wheat Haploid Plant through Wheat × Maize

作　　者：蔡华[1] 马传喜[2] 乔玉强[2] 陆维忠[3] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100 [2]安徽农业大学农学院,安徽合肥230036 [3]江苏省农科院遗传研究所,江苏南京210014

出　　处：《激光生物学报》2007年第1期43-48,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(30270825);安徽省教育厅自然科学研究项目(2006KJ142C);安徽省高校青年教师科研资助计划项目(2006jql198);安徽科技学院自然科学研究项目(2RC200547)

摘　　要：为获得一定规模的小麦单倍体植株以构建小麦DH(Double Haploid)群体,采用小麦(Triticum aestivum,2n=42)与玉米(Zeamays,2n=20)远缘杂交诱导小麦单倍体胚,经胚拯救产生单倍体植株,研究了1/2MS培养基中幼胚取材时期、幼胚大小、4℃处理时间、暗处理时间对单倍体胚培养再生成苗的影响,结果表明:授粉后12天~16天取材的幼胚经胚拯救后成苗率无明显差异;0.5mm^1.0mm大小的幼胚成苗率显著高于0mm^0.5mm和1.0mm^1.5mm大小的幼胚成苗率;1天~3天短期4℃处理对胚萌发具有一定促进作用,但处理3天后,出愈率和成苗率降低;胚培养过程中12天左右的24h暗处理能有效提高成苗率。In order to obtain a large number of wheat haploids to establish the DH（ Double Haploid） groups, wheat and maize cross were used to induce wheat haploid embryos and then the haploid plants were produced via young embryo culture. The factors affecting the regeneration of wheat young plants from young embryos, such as different period of peeling embryo,size and development stage of young embryos,the treatment time at 4 ℃ and dark treatment time were studied. The results showed ： there was no significant difference on the frequency of young plants production when haploid embryos was peeled at different time from 12 days to 16 days; the frequency of young plants production of the size of 0.5 mm -1.0 mm is much higher than that of the size of 0 mm - 0.5 mm and 1.0 mm - 1.5 mm ; the short time treatment between 1 day - 3 days at 4℃ would accelerate the regeneration of young embryo, but after 3 days the frequency of callus induction and plants production would decrease; dark treatment of all 24 h for 12 days could effectively improve the frequency of plants production during the process of young embryo culture.

关 键 词：小麦 玉米 胚培养 单倍体植株 成苗率 

分 类 号：Q813[生物学—生物工程] S512.2[农业科学—作物学]