葡多酚对小鼠肝细胞蛋白激酶C和增殖细胞核抗原表达的影响  

Effect of grape procyanidins on the protein kinase and proliferative cell nuclear antigen protein expression of mice hepatic cells

作　　者：刘辉[1] 钟进义[1] 于萍[1] 李蕾[1] 

机构地区：[1]青岛大学医学院,青岛266021

出　　处：《卫生研究》2007年第1期31-33,共3页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30271127)

摘　　要：目的观察葡多酚(GPC)对小鼠肝细胞蛋白激酶C(PKC)和增殖细胞核抗原(PCNA)表达的影响。方法每日给小鼠经口灌胃不同剂量的GPC,30天后取肝组织,用MTT法测各组小鼠肝细胞的增殖活性水平,免疫组化的方法检测各组PKC和PCNA的表达。结果高剂量GPC组PKC和PCNA阳性表达率分别为47.62%和82.76%,阴性对照组则分别为6.42%和32.62%,经χ2检验,差异有显著性(P<0.05)。各组肝细胞的PKC和PCNA阳性表达率随GPC剂量的增高而增高。结论葡多酚可促进小鼠肝细胞PKC的表达,提高肝细胞增殖活性。Objective To study the effect of grape procyanidins （GPC） on the protein kinase C（PKC） and proliferative cell nuclear antigen （PCNA） expression in mice hepatic cells. Methods The mice were orally given at the doses of GPC and killed after 30 days. The proliferation activity of the hepatic cells was determined by MTT assay, and the levels of PKC and PCNA protein expression in the cell were measured by immunohistochemistry method. Results The expression rates of PKC and PCNA protein of the high-dose GPC group were 47.62 % and 82.76 % respectively, and those of the negative control group were 6.42% and 32.62%. The differences between the two groups were significant（ P 〈 0.05 ）. The proliferation of mice hepatic cells increased with the dose of GPC in different groups. Conclusion GPC could promote the PKC protein expression and improve the proliferative activity of the mice hepatic cells.

关 键 词：葡多酚 肝细胞 蛋白激酶C 增殖细胞核抗原 增殖活性 

分 类 号：Q556.9[生物学—生物化学] Q946.82