人肝病变和热凝固导致组织的光穿透深度变化  

Pathological changes and thermal coagulation of human liver tissue induced changes in the optical penetration depths in vitro

作　　者：魏华江[1] 何博华[2] 陈雪梅[3] 许静芬[4] 张薇[4] 

机构地区：[1]华南师范大学激光生命科学研究所,广州510631 [2]广东药学院临床医学系外科,广州510224 [3]中山大学第一附属医院眼科,广州510080 [4]广东药学院电子物理学教研室,广州510224

出　　处：《激光技术》2007年第1期31-34,共4页Laser Technology

摘　　要：为了研究人肝的病变及热凝固导致其对532nm和1064nm激光的光穿透深度的变化，采用了双积分球和反向倍增法。实验结果为，肝癌组织对532nm的光穿透深度显著地较正常肝组织对532nm的光穿透深度要大（P〈0．05），肝癌组织对1064nm的光穿透深度却显著地较正常肝组织对1064nm的光穿透深度要小（P〈0．05），自然的正常肝组织和癌变的肝组织对1064nm的光穿透深度都分别显著地较其对532nm的光穿透深度要大（P〈0．05）；热凝固的正常肝组织对532nm和1064nm的光穿透深度都分别显著地较自然的正常肝组织对相应波长的光穿透深度要小（P〈0．05），热凝固的肝癌组织对532nm和1064nm的光穿透深度都分别显著地较自然的肝癌组织对相应波长的光穿透深度要小（P〈0．05），热凝固的正常的肝组织和癌变的肝组织对1064nm的光穿透深度都分别显著地较其对532nm的光穿透深度要大（P〈0．05）。此研究结果将有助于肝癌的光热治疗。In order to study the optical penetration depths of liver tissue at 532nm KTP laser and 1064nm Nd：YAG laser induced by pathological changes and thermal coagulation of human liver tissue in vitro, measurements were performed with a double-integrating-sphere setup and the inverse adding-doubling method. The measurement results showed that the optical penetration depth of cancerous liver tissues at 532nm was significantly bigger than that of normal liver tissues at the same wavelength（ P 〈 0.05 ）, but the optical penetration depth of cancerous liver tissues at 1064nm was significantly smaller than that of normal liver tissues at the same wavelength （ P 〈 0. 05 ） , the optical penetration depth of native normal and cancerous liver tissues at 1064nm was significantly bigger than that of native normal and cancerous liver tissues at 532nm, respectively （P 〈 0.05 ）. The optical penetration depth of coagulated tissues of normal liver at 532nm and 1064nm was significantly smaller than that of native tissues of normal liver at the corresponding wavelength, respectively （ P 〈 0.05 ） , however the optical penetration depth of coagulated tissues of cancerous liver at 532nm and 1064nm was significantly smaller than that of native tissues of cancerous liver at the corresponding wavelength （ P 〈 0.05 ）. The optical penetration depth of coagulated tissues of normal and cancerous liver at 1064nm was significantly smaller than that of coagulated tissues of normal and cancerous liver at 532nm, respectively（ P 〈 0.05 ）. The results of measurement axe helpfull to the treatment of liver cancer by light and heat.

关 键 词：医用光学与生物技术 光穿透深度 积分球 人肝组织 KTP/YAG激光 病变 热凝固 

分 类 号：R318.51[医药卫生—生物医学工程]