多杀菌素的发酵工艺研究  

Research on the Fermentation Conditions of Spinosad

作　　者：胡旭晔[1] 张琴[1] 胡海峰[1] 朱宝泉[1] 

机构地区：[1]上海医药工业研究院,上海200040

出　　处：《中国医药工业杂志》2007年第2期90-93,共4页Chinese Journal of Pharmaceuticals

摘　　要：用均匀设计法优化了多杀菌素发酵培养基的配方,同时探讨了前体物质对发酵效价的影响。结果得到优化培养基组成(g/L):葡萄糖37.8,糊精42.0,棉籽粉22.3,脱脂奶粉9.0,CaCO33.0。发酵30h后添加10mmol/L的正丁醇效果最佳,优化后多杀菌素的效价为394.1μg/ml,比优化前提高了56%。Uniform design method was employed to optimize the fermentation medium. Meanwhile, the effects of precursors on the biosynthesis of spinosad were also studied. Components of the optimized medium （g/L）were 37.8 glucose, 42.0 dextrin, 22.3 cottonseed flour, 9.0 skimmed milk powder and 3.0 calcium carbonate. The fermentation potency of spinosad could reach to 394.1μg/ml when flask fermentation by Saccharopolyspora spinosa sp-29 was carried out with shaking in the optimized medium feeding 10mmol/L n-butanol at 30h, which had increased by 56%.

关 键 词：多杀菌素 刺糖多孢菌 前体 均匀设计 

分 类 号：TQ460.38[化学工程—制药化工]