抗肿瘤肽AP-9的固相合成  

Solid-Phase Synthesis of Antitumor Peptide AP-9

作　　者：李佩华[1] 王卫国[1] 沈树宝[1] 欧阳平凯[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《中国医药工业杂志》2007年第2期93-96,共4页Chinese Journal of Pharmaceuticals

基　　金：国家863基金(2003AA219040;20060102Z2009);国家自然科学基金(20376034);江苏省自然科学基金(BK2003084)

摘　　要：建立了抗肿瘤肽AP-9的Fmoc固相全合成方法,产品纯度大于98%。着重研究了Fmoc-Arg(Pbf)-OH与H-Pro-Trt树脂的缩合反应。结果表明,以THF-NMP(1∶1)为溶剂,于30℃微波辐射反应6min,缩合产率达80.2%。Antitumor peptide AP-9 （YKLPGHHHHYRP） was synthesized by Fmoc-solid phase peptide synthesis, and the purity of the product was more than 98 %. The coupling reaction between the difficult sequence Fmoc- Arg （Pbf）-OH and H-Pro-Trt resin was investigated. The results showed that the coupling reaction could be completed when it was carried out for 6min at 30℃ by microwave irradiation with THF-NMP （1 ： 1） as the solvent, and the yield could reach to 80.2 %.

关 键 词：AP-9 抗肿瘤肽 固相合成 微波 

分 类 号：TQ464.7[化学工程—制药化工]