邻苯二甲酸二丁酯对体外人肝细胞HL-7702毒性作用的研究  

The Toxic Effects of Di-n-butyl Phthalate on Human Liver HL-7702 Cells

作　　者：李玲[1] 简英[1] 刘贺荣[1] 

机构地区：[1]宁夏医学院公共卫生学院,银川750004

出　　处：《宁夏医学院学报》2007年第1期38-40,共3页Journal of Ningxia Medical College

基　　金：宁夏医学院面上科研项目(校GW200522)

摘　　要：目的探讨邻苯二甲酸二丁酯对肝细胞的毒性作用及可能的损伤机制。方法1、10、50、100μmol/L浓度的邻苯二甲酸二丁酯作用于人肝细胞HL-7702后,检测邻苯二甲酸二丁酯对肝细胞HL-7702的增殖率、超氧化物歧化酶(SOD)、丙二醛(MDA)含量的变化。结果邻苯二甲酸二丁酯(DBP)作用于肝细胞后,肝细胞HL-7702的增殖率随DBP浓度的增加而下降,在≥10μmol/L浓度,细胞的增殖率显著降低(P<0.01),肝细胞的增殖率从对照组的100%降低到100μmol/L浓度的50.7%。DBP可使肝细胞HL-7702中的SOD含量降低,MDA含量升高,当浓度大于50μmol/L时,与对照组相比均具有统计学意义(P<0.01)。结论DBP对体外培养人肝细胞HL-7702具有细胞毒性和氧化损伤作用。Objective To study the toxic effects of Di-n-butyl phthalate on human liver HL - 7702 cells and the possible mechanisms. Methods Human liver HL- 7702 cells were exposed to different concentration of Di-n- butyl phthalate （1μmol/L, 10mol/l, 50mol/L, 100mol/L）. Proliferation rate and level of SOD, MDA were measured. Results After exposed to Di-n-butyl phthalate, HL - 7702 cell proliferation rate decreased when the concentration of Di-n-butyl phthalate increased. Di-n-butyl phthalate significantly decreased HL - 7702 cell proliferation rate at 10mol/L, Human Liver proliferation rate from 100% of contrast group to 50.7% of 100mol/L. Di-n-butyl phthalate significantly decreased SOD level and increased MDA level at 50mol/L. Conclusion Di- n-butyl phthalate has cytotoxic effect and oxidative damage to HL- 7702 cells.

关 键 词：邻苯二甲酸二丁酯 超氧化物歧化酶 肝细胞株 丙二醛 

分 类 号：R114[医药卫生—卫生毒理学]