引起不同临床表现的巨细胞病毒UL144基因多态性的研究  

Human cytomegalovirus UL144 gene polymorphism in enrly infant with different clinical manifestation

作　　者：毛志芹[1] 曾祥士[1] 王珺珺 阮强[3] 孙梅[1] 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004 [2]沈阳市和平区新兴社区卫生服务中心,110005 [3]中国医科大学附属盛京医院病毒室,沈阳110004

出　　处：《中国小儿急救医学》2007年第1期39-42,共4页Chinese Pediatric Emergency Medicine

基　　金：国家自然科学基金资助项目（30170986;30371492）.

摘　　要：目的研究不同临床背景的人巨细胞病毒（HCMV）临床株中UL144基因的多态性，探讨其多态性与HCMV感染临床表现之间的关系。方法采用巢式PCR法，对具有不同临床表现的122份HCMV感染患儿的尿标本和53份先天性巨结肠患儿痉挛段肠组织标本的临床株进行UL144开放阅读框的扩增，扩增阳性的临床株进行UL144开放阅读框核苷酸测序。结果50份尿标本和23份肠组织标本临床株完成DNA测序。种系进化树分析结果显示HCMVUL144基因分为3组4个基因型，UL144G1a（52％）为主要基因型。有症状与无症状HCMV感染临床株的基因型分布比较，差异无显著性。引起神经系统及肝胆系统受累的临床株与无症状感染临床株基因型分布比较，差异无显著性。与美国及日本的临床株比较，UL144基因型分布差异具有显著性。结论HCMVUL144基因具有高度多态性；UL144G1a为先天性或围生期HCMV感染的主要基因型；UL144基因分布与地理位置有关；UL144基因分型与HCMV感染的临床表现及组织嗜性无关。Objective To explore human cytomegalovirus （HCMV） UL144 gene polymorphism and investigate the relationship between HCMV UL144 gene polymorphism and clinical manifestations. Methods All the strains from 70 infants with suspected congenital and/or perinatal HCMV infection were non-passage strains. Among them, 23 strains were from surgical colon specimens, and the others were from urine samples. HCMV UL144 gene of 73 clinical strains from 70 infants were successfully amplified and sequenced. Results Thirty-six patients （49.3 % ） were infected with group G1 （Gla 33/36, Glb 3/36）, 19 patients （28.8%） were infected with group G2, and 15 patients （21.9%） were infected with group G3 of UL144 gene. Compared with the distribution pattern of strains in UL144 genotypes of data from Chicago, Iowa and Texas, and Japan by chi-square test, the difference was statistically significant. Conclusion The distribution pattern of UL144 genotype was associated with geographic location. However, UL144 genotype is not related to the severity and tissue tropism of HCMV disease.

关 键 词：人巨细胞病毒 基因UL144 多态性 

分 类 号：R72[医药卫生—儿科]