转化生长因子β1在大鼠窒息复氧心肌损伤后心肌重塑中的作用  

Correlation of transforming growth factor β1 and myocardial remodeling following anoxia-reoxygenation injury in the rats

作　　者：徐书珍[1] 赵炜[1] 王彩霞[1] 初建芳[1] 

机构地区：[1]山东省青岛市市立医院小儿科,266011

出　　处：《中国小儿急救医学》2007年第1期48-50,F0004,共4页Chinese Pediatric Emergency Medicine

摘　　要：目的观察大鼠窒息复氧心肌损伤后心肌内源性转化生长因子（TGF）-β1表达，探讨TGF-β1在心肌缺氧缺血再灌注损伤修复及心肌重塑中的作用。方法制作窒息复氧心肌损伤大鼠动物模型，将60只新生大鼠随机分成窒息复氧心肌损伤1d组（A组）、7d组（B组）、14d组（C组）以及正常对照组（D组）。每组15只，快速定量检测血清心肌肌钙蛋白（cTnI）水平，心肌TGF-β1表达采用免疫组化方法进行定性及半定量分析，用Masson染色方法测定心肌总胶原容积（CVF），同时利用HE染色观察心肌组织的病理学改变。结果血清cTnI呈一过性增高，A组明显高于D组，差异非常显著（P〈0．01），B、C组下降，与D组比较差异无显著性（P〉0．05）。TGF-β1表达及CVF随复氧时间延长而增加，A组轻度增高，但较D组差异无显著性（P〉0．05），B组明显增高，C组达高峰，与D组比较差异均非常显著（P〈0．01）。CVF与TGF-β1表达呈正相关（r=0．574，P〈0．01）。结论大鼠窒息复氧心肌损伤后，TGF-β1表达随复氧时间的不同而变化，可能参与了心肌损伤后的自我修复及心肌重塑。Objective To observe the expression of endogenous transforming growth factor β1（TGF- β1 ）and its effect on myocardial repairing and remodeling after anoxia- reoxygenation injury in rats. Methods The model of myocardial anoxia- reoxygenation injury was established. Sixty neonatal rats were randomly divided into four groups（15 rats in each group）：1 day（group A）,7 days（group B）and 14 days（group C）after myocardial anoxia- reoxygenation injury and normal control group（group D）. The serum cardiac troponin（cTnI） level was quantified by a rapid method. The TGF-β1 expression of myocardium was measured by immunohistochemical assay. Masson staining was used to study the total collagen volume fraction （CVF）. Results Serum cTnI level showed transient increase and the level of group A was obviously higher than that of group D （ P 〈 0.01）, but the level of group B and group C declined, there were not significant difference in comparison to group D （P 〉0.05）. The expression level of TGF-β1 and CVF in group A increased lightly, but obviously increased in group B and peaked in group C, there were very significant difference in comparison to group D （ P 〈 0.01 ）. CVF was positively correlated with the expression level of TGF-β1 （ r = 0. 574, P 〈 0.01 ）. Conclusion The expression level of TGF-β1 in myocardium is different at the different time points after anoxia-reoxygenation injury, which possibly plays an important role in myocardial self-repairing and remodeling.

关 键 词：窒息复氧心肌损伤 心肌重塑 转化生长因子Β1 大鼠 

分 类 号：R54[医药卫生—心血管疾病]