与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发  

Development of SCAR Markers Linked to Resistance Locus of Black Spot Disease in Populus deltoides

作　　者：张燕梅[1] 潘惠新[1] 张博[1] 王明庥[1] 黄敏仁[1] 

机构地区：[1]国家林业局江苏省林木遗传和基因工程重点实验室(南京林业大学),江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2007年第1期15-18,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金重点项目(30230300)

摘　　要：通过测序和引物设计,将与美洲黑杨抗黑斑病基因相连锁的RAPD标记OPAI17-1550和OPAI13-900成功地转化成显性SCAR标记(SAI17-1570)和共显性SCAR标记(SAI13-292),并对感、抗病池和F1代91个无性系进行了SCAR标记检测。SAI17-1570在抗病池和OPAI17-1550阳性的F1代植株中扩增出1条与RAPD扩增结果相符的特异性条带,而在感病池和OPAI17-1550阴性的F1代植株中没有相应的带,SAI13-292在抗病池和OPAI13-900阳性的F1代植株中扩增出2条带,而在感病池和OPAI13-900阴性的F1代植株中只有其中1条带。RAPD markers OPAI17-1550 and OPAI13-900, linked to anti-black-spot disease gene locus, were successfully converted into SCAR markers named SAI17-1570 and SAI13 -292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13-292 were validated in the resistant.susceptible pools and F1 population. The SAI17-1570 had the same amplification with templates of the positive OPAI17-1550 F1 population and resistant pool, did not have amplification with templates of the negative OPAI17-1550 F1 population and susceptible pool. The SAI13-292 had one band with templates of the negative OPAI13-900 F1 population and susceptible pool, but two bands with templates of the positive OPAI13-900 F1 population and resistant pool. The two SCAR markers could be of great importance for clone selection on seedling stage.

关 键 词：杨树黑斑病 随机扩增多态性DNA 序列特异性扩增区域 

分 类 号：S722[农业科学—林木遗传育种] Q813[农业科学—林学]