杂交鹅掌楸花粉败育的结构变异与分析  

The Analysis for the Variant Structure of Abortive Liriodendron chinense×L.tulipifera Pollen

作　　者：徐柏森[1] 何开跃[1] 张耀丽[2] 王章荣[1] 彭冶[1] 甘习华[1] 杨静[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037 [2]南京林业大学木材工业学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2007年第1期47-50,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：江苏省普通高校自然科学研究计划资助项目(03KJB220049)

摘　　要：应用透射电子显微镜研究了杂交鹅掌楸花粉的超微结构形态,并对其花芽发育过程中相关的生理指标做了测定。结果显示:在花药发育初期,败育小孢子母细胞形态异常,细胞质凝聚,细胞器结构模糊,失去细胞分裂功能,不能进行减数分裂。发育正常的小孢子母细胞形态呈圆状或椭圆状,其中细胞质丰富、细胞器结构正常的小孢子母细胞发育成熟后,进入减数分裂形成四分体;而细胞质不丰富或局部细胞器结构退化的小孢子母细胞,虽然可以进入减数分裂,但很难完成,不能形成正常的四分体。同时杂交鹅掌楸花粉的绒毡层细胞形态异常,也造成了花药发育停滞、细胞解体、黏连堆积和畸形生长。这与生理指标中显示的花芽在发育过程中RNA含量、蛋白质含量、氨基酸总量和SOD活性呈下降趋势的结果一致。The ultra-structure of Liriodendron pollen was studied by transmission electron chinense （Hemsl. ） Sarg. × L. tulipifera L. microscope. Correlative physiological indexes were determined during the development of bud. The results showed that the form of abortive microspore mother cell was abnormal, the cytoplasm was condensed and the structure of organelle was fuzzy at the initial stage of anther development. Then it lost division function and couldn＇t under go meiosis. Generally normal microspore mother cells were round or oval. The cell whose cytoplasm was abundant and organelle structure was clear could complete the normal meiosis. Contrarily,it only could enter meiosis but not finish it. So the normal tetrad didn＇t come into being. And the deformed tapetum cell caused stasis of anther development, disintegrating of cells, fusing, piling up and deforming. At the same time, the content of RNA, protein and amino acids reduced constantly in the course of stamen development.

关 键 词：杂交鹅掌楸 超微结构 花粉败育 透射电镜 

分 类 号：S722[农业科学—林木遗传育种] S792.21[农业科学—林学]