酶改良免疫组化检测PSA等四项指标联合诊断前列腺癌的价值  

Differential diagnosis of prostate cancer by modified immunohistochemistry of prostate specific antigen,P63,high molecular weight cytokeratin and P504S

作　　者：林虹[1] 姜汉国[1] 吴冬梅[1] 林琼燕[1] 

机构地区：[1]广东医学院附属医院,广东湛江524001

出　　处：《宁夏医学杂志》2007年第2期106-108,共3页Ningxia Medical Journal

摘　　要：目的探讨前列腺特异性抗原(PSA)、高分子量细胞角蛋白(CK34βE12)、P63和支链脂肪酸β氧化酶(P504S)改良免疫组化对前列腺癌鉴别诊断的作用。方法对92例前列腺癌(PA)、20例良性前列腺增生(BPH)和20例前列腺上皮内瘤(PIN)石蜡标本采用改良免疫组化染色法检查。即在同一侧贴附可靠的阳性对照组织,应用高压抗原修复法处理,尽可能保存和修复抗原,在显微镜下严格控制显色等方法以达到最佳染色效果并结合HE标本观察结果。结果PSA、CK34βE12、P63和P504S在BPH、PIN和PA中的阳性表达分别为70.0%(14/20)、80%(16/20)6、6.3%(61/92),100.0%(20/20)、80%(16/20)、5.4%(5/92),100.0%(20/20)、90%(18/20)、3.3%(3/92),10.0%(2/20)、25%(5/20)、85.7%(79/92)。3组间CK34βE12、P63和P504S阳性表达率有非常显著性差异(P<0.01)。CK34βE12和P63阳性表达与列腺腺泡腺癌的Gleason分级评分之间密切相关。膀胱尿路上皮癌PSA和CK34βE12呈阴性表达。结论PSA、P63、P504S和CK34βE12改良的免疫组化法和抗体联合染色应用可作为前列腺癌诊断和鉴别诊断的重要指标。Objective To evaluate the differential diagnostic value of prostate specific antigen（PSA） , P63, high molecular weight cytekeratin （CK34βE12）and P504S to prostate carcinoma （PA） .Methods An irnmunohistochemical method for PSA ,P63 ,CK34βE12 and P504S was modified as follows ： （1） A reliably positive control tissue section was adhered beside the tested tissue section on the same slide ;（2） The slide was treated with microwave oven to preserve and repair the antigens and （3） Developing the colour of sections must be controlled microscopically until the background was clear. Results The positive expression rates of PSA, CK34βE12, P63 and P504S 70.0% （ 14120, 80% （16/20） ,66.3 % （61/92）, 100.0% （20/20,80% （ 16/20）, 5.4% （5/92）, 100.0% （20/20）, 90% （ 18/20,3.3% （3/92）, and 10.0% （2/20）, 25 % （ 5/20 ）, 85.7 % （79/92） respectively. Positive rates of P63, CK34βE12 and P504S were significantly different （ P 〈 0.01 ） among three groups. Positive rates of P63 and CK34βE12 had close relation with Gleason histological grading of prostate carcinoma. PSA and CK34βE12 were not expressed in transitional cell carcinoma.Conclusion Modified immunohistochemistry and combined detection of PSA, P63, CK34βE12 and P504S can be most helpful for diagnosis and differential diagnosis of prostrates carcinoma.

关 键 词：前列腺特异性抗原 P63 高分子量细胞角蛋白 支链脂肪酸β氧化酶 

分 类 号：R446.62[医药卫生—诊断学]