舟山眼镜蛇毒蕈碱样多肽的分离及其对M胆碱受体的激动效应  

The Agonistic Effect of Muscarinic Peptide Isolated from Naja atra Venom to Muscarinic Cholinergic Receptor

作　　者：郑佳冰[1] 黄立峰[1] 许盈[1] 俞昌喜[1] 

机构地区：[1]福建医科大学药学院,福州350004

出　　处：《福建医科大学学报》2007年第1期1-4,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目(C0410018);福建医科大学教授学术发展基金(JS06027);福建省重点高校建设科研基金重点项目(XZ04008)

摘　　要：目的从舟山眼镜蛇Naja atra蛇毒中分离毒蕈碱样多肽,探讨其与M胆碱受体的关系。方法应用分子筛凝胶Sephadex G-50、Sephadex G-100、离子交换凝胶CM-Sepharose F.F.分离纯化目的组分;SDS-PAGE鉴定目的蛋白的纯度并测定其相对分子质量;放射受体分析法测定该物质与大鼠脑组织M胆碱受体的亲和力;选用离体豚鼠回肠纵行肌观察其对M胆碱受体的效应。结果从舟山眼镜蛇蛇毒中获得一种与大鼠脑组织M胆碱受体具有高亲和力的多肽(Ki为10.12 nmol/L),其相对分子质量约14 kD。该多肽可引起离体豚鼠回肠纵行肌收缩,此效应可被阿托品阻断。结论舟山眼镜蛇蛇毒中获得与M胆碱受体具有高亲和力的多肽,该多肽对M胆碱受体具有激动效应。Objective To isolate muscarinic peptide（MP） from Naja atra venom and tO study the effect of MP in muscarinic cholinergic receptor（mChR）. Methods MP had been isolated and purified from Naja atra venom by gel filtration on Sephadex G-50 and Sephadex G-100, chromatography on ion-exchanger CM-Sepharose FF.Homogenicity and molecular weight of the MP was determined on SDS-PAGE. Isolated ileal longitudinal muscles of the guinea pigs were used to observe the effect of MP on mChR. The affinity of MP to rat brain synaptosomal membrane was tested by radio receptor assay. Results Muscarinic peptide isolated from Naja atra venom could displace [^3 H]-QNB from rat brain. Its molecular weight was 14 kD. In guinea-pig ileum, MP produced a potent contraction which can be blocked by atropine. Conclusion The muscarinic peptide from Naja atra venom was muscarinic agonist.

关 键 词：眼镜蛇毒液类 受体 胆碱能 毒蕈碱 胆碱能激动剂 放射受体分析法 

分 类 号：R285[医药卫生—中药学]