SLE患者外周血CD4^+CD25^+调节性T细胞及相关基因Foxp3的变化  

The changes of CD4^+CD25^+ T cells and expression of Foxp3 mRNA in peripheral blood mononuclear cell from patients with systemic lupus erythematosus

作　　者：卢雪红[1] 于春雷[1] 李一[1] 

机构地区：[1]吉林大学白求恩医学部基础医学院免疫学教研室,吉林长春130021

出　　处：《中国老年学杂志》2007年第2期99-101,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助课题(30571724)

摘　　要：目的研究系统性红斑狼疮(SLE)合并狼疮性肾炎患者经肾上腺糖皮质激素(简称激素)冲击治疗后,外周血CD4+CD25+调节性T细胞(regulatoryTcell,Treg)及相关基因Foxp3表达的变化,从而探讨CD4+CD25+Treg和Foxp3与SLE发病的相关性。方法采用流式细胞术检测SLE患者外周血单个核细胞(PBMC)中CD4+CD25+T、CD4+CD25highT细胞数量的变化,RT-PCR检测PBMC中CD4+CD25+Treg功能相关基因Foxp3mRNA的表达。结果激素冲击治疗后的SLE患者CD4+CD25+T/CD4+T及CD4+CD25highT/CD4+T比值高于正常对照组;Foxp3mRNA水平表达与对照组差异不显著。结论CD4+CD25+Treg数量和功能的变化可能参与SLE的发病,激素可能通过提升CD4+CD25+Treg治疗SLE。Objective To investigate if CD4^＋ CD25^＋ regulator T cells （Treg） and transcription factor Foxp3 is related with systemic lupus erythematosus （SLE） by quantify CD4^＋ CD25^＋ regulatory T cells in peripheral blood from SLE patients after treatment by high-dose glucocorticoids. Methods Flow cytometry was used to analyze the proportion of CD4^＋ CD25 ^＋ T and CD4^＋ CD25highT cells in peripherallblood mononuclear cells （PBMC）. The expression of Foxp3 mRNA in PBMC was detected by RT-PCR. Results After treatment, the proportion of CD4^＋ CD25^＋ T/CD4^＋ Tcells and CD4^＋ CD25highT/CD4 ^＋ Tcells were significantly higher in SLE group than those in control group （ P 〈 0.05 ）, but the expression of Foxp3 mRNA had no difference between SLE patients and controls （ P 〉 0.05）. Conclusions The changes of CD4^＋ CD25^＋ Treg cell in number and function might participate in onset of SLE. The glucocorticoid may increase CD4^＋ CD25 ^＋ Treg frequency to treat SLE.

关 键 词：CD4^+CD25^+T细胞 FOXP3 系统性红斑狼疮 

分 类 号：R593.241[医药卫生—内科学]