实验性糖尿病大鼠内皮屏障抗原表达和血脑屏障通透性变化  

The effects of diabetes on EBA expression and blood-brain barrier permeability in rat

作　　者：尹岭[1] 刘式威[1] 吕霞 张梅奎[1] 杜侃[1] 

机构地区：[1]解放军总医院神经信息中心,北京100853 [2]解放军第六五四二六部队医院

出　　处：《中华神经科杂志》2007年第1期47-49,共3页Chinese Journal of Neurology

基　　金："973"国家重点基础研究发展计划资助项目(2004CB720302)

摘　　要：目的研究糖尿病对血脑屏障(BBB)通透性和内皮屏障抗原(EBA)表达的影响。方法雄性 SD 大鼠20只,随机分为正常对照组和糖尿病组。用链脲霉素(streptozotocin,50 mg/kg)静脉一次性注射制备糖尿病模型。4个月后取两组大鼠前额叶皮质,用免疫组织化学的方法检测微血管内皮细胞 EBA 及微血管周围纤维蛋白原(fibrinogen)的表达。结果免疫组织化学染色显示,糖尿病组EBA 阳性微血管数(11.30±1.15)与正常对照组(17.20±1.03)比较明显减少(P<0.05)。糖尿病组微血管周围纤维蛋白原呈弱阳性表达(5.80±1.98),正常对照组为阴性表达。结论糖尿病可引起EBA 表达减少,BBB 通透性增加;EBA 表达减少可能是糖尿病引起 BBB 通透性增加的一个重要因素。Objective To investigate the effects of diabetes on endothelial barrier antigen （EBA） expression and blood-brain barrier （BBB） permeability. Methods Twenty male SD rats were randomly divided into normal control and diabetic groups. SD rats were made diabetic by intravenous streptozotocin （50 mg/kg ） injection. After 4 months, The rat prefrontal cortex was removed. Employing immunohistochemical （IHC） staining the relative distribution was determined for the EBA and extravasated fibrinogen around microvessels. Results By IHC method, the number of EBA positive microvessels was significantly decreased for diabetic group （ 11.30 ± 1. 15 ） compared with that of control group （ 17. 20 ± 1.03, P 〈 0. 05 ）. The expression of fibrinogen was positive around microvessels for diabetic group （5. 80 ± 1.98）, but negative for control group. Conclusions Diabetes may reduce EBA expression and increase BBB permeability. Decreased EBA expression may be an important means by which diabetes causes increased BBB permeability.

关 键 词：血脑屏障 糖尿病 实验性 抗原 纤维蛋白原 免疫组织化学 

分 类 号：R587.1[医药卫生—内分泌]