鲁西黄牛α干扰素成熟蛋白基因的克隆及序列分析  

Cloning and Sequencing Analysis of Luxi Yellow Cattle IFN-α Gene

作　　者：张永红[1] 王长法[2] 杨少华[2] 高运东[2] 王洪梅[2] 李景鹏[1] 仲跻峰[2] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]山东省农业科学院奶牛研究中心,山东济南250100

出　　处：《动物医学进展》2007年第2期12-14,共3页Progress In Veterinary Medicine

基　　金：山东省"三○"工程资助(2003-3009)

摘　　要：根据GenBank上发表的牛α干扰素基因序列,设计了一对特异性引物。提取黄牛血液中白细胞基因组DNA,PCR扩增出α干扰素基因,并与pMD18-T克隆载体相连。测序结果表明,扩增片段含有498个核苷酸,编码166个氨基酸的成熟蛋白,与已报道的牛α干扰素C亚型最相近,氨基酸序列同源性为97.6%。获得BoIFNα-成熟蛋白基因,为重组牛干扰素的开发奠定了基础。One pair of specific primers of bovine interferon α gene was designed and synthesized according to the published sequence in GenBank. Interferon α gene was cloned from genomie DNA of Chinese Luxi yellow cattle by PCR, and the PCR product was inserted into vector pMD18-T to construct plasmid pMD18-T/BoIFN-α. Sequence analysis showed that the Chinese Luxi yellow cattle IFN-α gene was composed of 498 nueleotides, eneoding a mature polypeptide with 166 amino aeids. Compared with other BoIFN-α subtypes, the amino aeid homology was 97.6 %. The results showed that the eloning plasmid was suecessfully eonstrueted and it laid a foundation for the produetion and development of Luxi yellow cattle interferon α gene.

关 键 词：鲁西黄牛 α干扰素基因 克隆 

分 类 号：Q78[生物学—分子生物学] S852.4[农业科学—基础兽医学]